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Liquid chromatographic determination of S-adenosyl-L-methionine in dietary supplement tablets

Liquid chromatographic determination of S-adenosyl-L-methionine in dietary supplement tablets
Liquid chromatographic determination of S-adenosyl-L-methionine in dietary supplement tablets
A simple and reliable liquid chromatographic method was developed for the determination of S-adenosyl-L-methionine (SAM) in dietary supplement tablets. SAM in products was extracted with a phosphate buffer and separated from the mixture on a reversed-phase C8 column by ion-pair chromatography. A gradient mobile phase containing phosphate buffer, sodium octanesulfonate as the ion-pair reagent, and acetonitrile at a flow rate of 1.2 mL/min was used in the analysis. The UV detection wavelength was set at 257 nm. The calibration curve was linear over a range of 75–375 ?g/mL for the SAM active ion with R2 = 0.9999. Replicate tests indicated good reproducibility of the method with a relative standard deviation of 0.9% (n = 8). The multiple extractions and recoveries from fortified products showed the high accuracy of the analysis. The use of the acidic buffer for SAM extraction and elution and the use of a fresh standard for each calibration to counteract the instability of the SAM compound significantly improved the accuracy of the method.
1060-3271
901-905
Zhou, Joseph Ziqi
876ad98d-b8e1-486b-a40d-27b201407202
Waszkuc, Ted
c0786c05-e80a-4e23-b1ad-3063136821f0
Garbis, Spiros
7067fd19-50c9-4d42-9611-f370289470bd
Mohammed, Felicia
c7f14743-5728-4c5b-835b-ede37cf01007
Zhou, Joseph Ziqi
876ad98d-b8e1-486b-a40d-27b201407202
Waszkuc, Ted
c0786c05-e80a-4e23-b1ad-3063136821f0
Garbis, Spiros
7067fd19-50c9-4d42-9611-f370289470bd
Mohammed, Felicia
c7f14743-5728-4c5b-835b-ede37cf01007

Zhou, Joseph Ziqi, Waszkuc, Ted, Garbis, Spiros and Mohammed, Felicia (2002) Liquid chromatographic determination of S-adenosyl-L-methionine in dietary supplement tablets. Journal of the AOAC International, 85 (4), 901-905. (PMID:12180685)

Record type: Article

Abstract

A simple and reliable liquid chromatographic method was developed for the determination of S-adenosyl-L-methionine (SAM) in dietary supplement tablets. SAM in products was extracted with a phosphate buffer and separated from the mixture on a reversed-phase C8 column by ion-pair chromatography. A gradient mobile phase containing phosphate buffer, sodium octanesulfonate as the ion-pair reagent, and acetonitrile at a flow rate of 1.2 mL/min was used in the analysis. The UV detection wavelength was set at 257 nm. The calibration curve was linear over a range of 75–375 ?g/mL for the SAM active ion with R2 = 0.9999. Replicate tests indicated good reproducibility of the method with a relative standard deviation of 0.9% (n = 8). The multiple extractions and recoveries from fortified products showed the high accuracy of the analysis. The use of the acidic buffer for SAM extraction and elution and the use of a fresh standard for each calibration to counteract the instability of the SAM compound significantly improved the accuracy of the method.

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Published date: July 2002
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 395430
URI: https://eprints.soton.ac.uk/id/eprint/395430
ISSN: 1060-3271
PURE UUID: 823c6caf-4c2c-4d74-a5a5-ded4e403c000
ORCID for Spiros Garbis: ORCID iD orcid.org/0000-0002-1050-0805

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Date deposited: 18 Jul 2016 12:49
Last modified: 20 Jul 2019 00:45

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Contributors

Author: Joseph Ziqi Zhou
Author: Ted Waszkuc
Author: Spiros Garbis ORCID iD
Author: Felicia Mohammed

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