Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry
Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry
Thiazide-based diuretics are included in the list of banned drugs in the horse-racing industry. One effect of their misuse is increased urine flow, contributing to dilution of other doping agents. Their determination is essential in ensuring compliance to horse-racing regulation. This study evaluates the feasibility of using liquid chromatography/mass spectrometry (LC/MS) with electrospray and atmospheric pressure chemical ionization interfaces to analyze thiazidic diuretics in equine urine samples. Existing LC and gas chromatography/MS methods are limited in their applicability to thiazide analysis. Sample preparation, analyte extraction, chromatographic separation, ion-source collision induced dissociation, solvent composition, ionization mode, and ion polarity are discussed. The practicality of LC/MS for this analysis is demonstrated with actual equine administration samples collected at specified time intervals. Detection limits were 270 ng/mL for chlorothiazide, 131 ng/mL for hydrochlorothiazide, and 384 ng/mL for trichlormethiazide.
948-957
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd
Hanley, L.
d24f72d3-afc9-4aaf-8f09-a690ae8c1fec
Kalita, S.
ab606088-49d0-48f7-9bd1-4c18d5cb7a45
September 1998
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd
Hanley, L.
d24f72d3-afc9-4aaf-8f09-a690ae8c1fec
Kalita, S.
ab606088-49d0-48f7-9bd1-4c18d5cb7a45
Garbis, S.D., Hanley, L. and Kalita, S.
(1998)
Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry.
Journal of the AOAC International, 81 (5), .
(PMID:9772736)
Abstract
Thiazide-based diuretics are included in the list of banned drugs in the horse-racing industry. One effect of their misuse is increased urine flow, contributing to dilution of other doping agents. Their determination is essential in ensuring compliance to horse-racing regulation. This study evaluates the feasibility of using liquid chromatography/mass spectrometry (LC/MS) with electrospray and atmospheric pressure chemical ionization interfaces to analyze thiazidic diuretics in equine urine samples. Existing LC and gas chromatography/MS methods are limited in their applicability to thiazide analysis. Sample preparation, analyte extraction, chromatographic separation, ion-source collision induced dissociation, solvent composition, ionization mode, and ion polarity are discussed. The practicality of LC/MS for this analysis is demonstrated with actual equine administration samples collected at specified time intervals. Detection limits were 270 ng/mL for chlorothiazide, 131 ng/mL for hydrochlorothiazide, and 384 ng/mL for trichlormethiazide.
Text
9772736_0-2.ILinkListener-search-recentsearches
- Other
Restricted to Repository staff only
Request a copy
Text
9772736_0-5.ILinkListener-search-recentsearches
- Other
Restricted to Repository staff only
Request a copy
Text
9772736;jsessionid=hgH0mguvI0BUbrSuoirY.0_0-1.ILinkListener-search-recentsearches
- Other
Restricted to Repository staff only
Request a copy
Text
9772736_0-4.ILinkListener-search-recentsearches
- Other
Restricted to Repository staff only
Request a copy
Text
9772736_0-3.ILinkListener-search-recentsearches
- Other
Restricted to Repository staff only
Request a copy
Text
9772736_0-6.ILinkListener-search-recentsearches
- Other
Restricted to Repository staff only
Request a copy
Show all 7 downloads.
More information
Published date: September 1998
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 395433
URI: http://eprints.soton.ac.uk/id/eprint/395433
ISSN: 1060-3271
PURE UUID: 9ca350e7-9b46-4091-8688-0c7d256e71e2
Catalogue record
Date deposited: 18 Jul 2016 13:00
Last modified: 15 Mar 2024 00:40
Export record
Contributors
Author:
S.D. Garbis
Author:
L. Hanley
Author:
S. Kalita
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
Loading...
View more statistics