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Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry

Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry
Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry
Thiazide-based diuretics are included in the list of banned drugs in the horse-racing industry. One effect of their misuse is increased urine flow, contributing to dilution of other doping agents. Their determination is essential in ensuring compliance to horse-racing regulation. This study evaluates the feasibility of using liquid chromatography/mass spectrometry (LC/MS) with electrospray and atmospheric pressure chemical ionization interfaces to analyze thiazidic diuretics in equine urine samples. Existing LC and gas chromatography/MS methods are limited in their applicability to thiazide analysis. Sample preparation, analyte extraction, chromatographic separation, ion-source collision induced dissociation, solvent composition, ionization mode, and ion polarity are discussed. The practicality of LC/MS for this analysis is demonstrated with actual equine administration samples collected at specified time intervals. Detection limits were 270 ng/mL for chlorothiazide, 131 ng/mL for hydrochlorothiazide, and 384 ng/mL for trichlormethiazide.
1060-3271
948-957
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd
Hanley, L.
d24f72d3-afc9-4aaf-8f09-a690ae8c1fec
Kalita, S.
ab606088-49d0-48f7-9bd1-4c18d5cb7a45
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd
Hanley, L.
d24f72d3-afc9-4aaf-8f09-a690ae8c1fec
Kalita, S.
ab606088-49d0-48f7-9bd1-4c18d5cb7a45

Garbis, S.D., Hanley, L. and Kalita, S. (1998) Detection of thiazide-based diuretics in equine urine by liquid chromatography/mass spectrometry. Journal of the AOAC International, 81 (5), 948-957. (PMID:9772736)

Record type: Article

Abstract

Thiazide-based diuretics are included in the list of banned drugs in the horse-racing industry. One effect of their misuse is increased urine flow, contributing to dilution of other doping agents. Their determination is essential in ensuring compliance to horse-racing regulation. This study evaluates the feasibility of using liquid chromatography/mass spectrometry (LC/MS) with electrospray and atmospheric pressure chemical ionization interfaces to analyze thiazidic diuretics in equine urine samples. Existing LC and gas chromatography/MS methods are limited in their applicability to thiazide analysis. Sample preparation, analyte extraction, chromatographic separation, ion-source collision induced dissociation, solvent composition, ionization mode, and ion polarity are discussed. The practicality of LC/MS for this analysis is demonstrated with actual equine administration samples collected at specified time intervals. Detection limits were 270 ng/mL for chlorothiazide, 131 ng/mL for hydrochlorothiazide, and 384 ng/mL for trichlormethiazide.

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More information

Published date: September 1998
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 395433
URI: https://eprints.soton.ac.uk/id/eprint/395433
ISSN: 1060-3271
PURE UUID: 9ca350e7-9b46-4091-8688-0c7d256e71e2
ORCID for S.D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805

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Date deposited: 18 Jul 2016 13:00
Last modified: 07 Jun 2019 00:32

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Contributors

Author: S.D. Garbis ORCID iD
Author: L. Hanley
Author: S. Kalita

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