Proteomic analysis of liver from transgenic mice overexpressing small heterodimer partner
Proteomic analysis of liver from transgenic mice overexpressing small heterodimer partner
The small heterodimer partner (SHP) is a key regulator of genes involved in cholesterol-bile acid homeostasis and functions as a specific transcription repressor. Differential protein expression in the liver of transgenic mice expressing the human SHP gene was compared with wild-type animals. Liver protein extracts were analyzed by two-dimensional electrophoresis and the proteins were identified by MALDI-TOF-MS. Approximately 30 proteins were differentially-expressed in the livers of transgenic mice, compared to the control mice. Major effects were evident in lipid accumulation, including a fatty acid-binding protein. Overexpression of SHP also triggered alterations in key enzymes involved in the metabolism of amino acids, nucleic acids and urea and was associated with changes in cellular proteins involved in calcium homeostasis, detoxification and protein folding and repair.
119-126
Kouyianou, Kallia
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Garbis, Spiros
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Boulais, Konstantinos
9944ad73-da18-456b-8feb-2d0539e0278b
Dimitraki, Ploumisti
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Fountoulakis, Michael
7918e434-8d49-49da-bd7e-45f71b6401b8
Tsiotis, Georgios
841903b7-c91e-46aa-8e92-b8de827cb2c2
March 2006
Kouyianou, Kallia
fd65f221-18c6-4828-84c1-37841fcb0b35
Garbis, Spiros
7067fd19-50c9-4d42-9611-f370289470bd
Boulais, Konstantinos
9944ad73-da18-456b-8feb-2d0539e0278b
Dimitraki, Ploumisti
211f535d-c39b-4ef3-95b2-f4b4d5bb0417
Fountoulakis, Michael
7918e434-8d49-49da-bd7e-45f71b6401b8
Tsiotis, Georgios
841903b7-c91e-46aa-8e92-b8de827cb2c2
Kouyianou, Kallia, Garbis, Spiros, Boulais, Konstantinos, Dimitraki, Ploumisti, Fountoulakis, Michael and Tsiotis, Georgios
(2006)
Proteomic analysis of liver from transgenic mice overexpressing small heterodimer partner.
Cancer Genomics Proteomics, 3 (2), .
Abstract
The small heterodimer partner (SHP) is a key regulator of genes involved in cholesterol-bile acid homeostasis and functions as a specific transcription repressor. Differential protein expression in the liver of transgenic mice expressing the human SHP gene was compared with wild-type animals. Liver protein extracts were analyzed by two-dimensional electrophoresis and the proteins were identified by MALDI-TOF-MS. Approximately 30 proteins were differentially-expressed in the livers of transgenic mice, compared to the control mice. Major effects were evident in lipid accumulation, including a fatty acid-binding protein. Overexpression of SHP also triggered alterations in key enzymes involved in the metabolism of amino acids, nucleic acids and urea and was associated with changes in cellular proteins involved in calcium homeostasis, detoxification and protein folding and repair.
Text
Proteomic Analysis of Liver from Transgenic Mice Overexpressing Small Heterodimer Partner.pdf
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Accepted/In Press date: 17 February 2006
Published date: March 2006
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 395449
URI: http://eprints.soton.ac.uk/id/eprint/395449
ISSN: 1109-6535
PURE UUID: 0dcdd66d-3c91-4c3b-b010-3fe2269d58ff
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Date deposited: 18 Jul 2016 13:48
Last modified: 15 Mar 2024 00:40
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Contributors
Author:
Kallia Kouyianou
Author:
Spiros Garbis
Author:
Konstantinos Boulais
Author:
Ploumisti Dimitraki
Author:
Michael Fountoulakis
Author:
Georgios Tsiotis
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