The University of Southampton
University of Southampton Institutional Repository

Surfactant Protein-D is essential for immunity to helminth infection

Surfactant Protein-D is essential for immunity to helminth infection
Surfactant Protein-D is essential for immunity to helminth infection
Pulmonary epithelial cell responses can enhance type 2 immunity and contribute to control of nematode infections. An important epithelial product is the collectin Surfactant Protein D (SP-D). We found that SP-D concentrations increased in the lung following Nippostrongylus brasiliensis infection; this increase was dependent on key components of the type 2 immune response. We carried out loss and gain of function studies of SP-D to establish if SP-D was required for optimal immunity to the parasite. N. brasiliensis infection of SP-D-/- mice resulted in profound impairment of host innate immunity and ability to resolve infection. Raising pulmonary SP-D levels prior to infection enhanced parasite expulsion and type 2 immune responses, including increased numbers of IL-13 producing type 2 innate lymphoid cells (ILC2), elevated expression of markers of alternative activation by alveolar macrophages (alvM) and increased production of the type 2 cytokines IL-4 and IL-13. Adoptive transfer of alvM from SP-D-treated parasite infected mice into naïve recipients enhanced immunity to N. brasiliensis. Protection was associated with selective binding by the SP-D carbohydrate recognition domain (CRD) to L4 parasites to enhance their killing by alvM. These findings are the first demonstration that the collectin SP-D is an essential component of host innate immunity to helminths.
1553-7366
1-18
Thawer, Sumaiyya
c595802c-3ced-4578-a07e-9453233a6ba6
Auret, Jennifer
255c6da8-b1ad-4ccb-87c8-cfe99a142a91
Schnoeller, Corinna
f6a29996-68f4-4798-94e2-c5d15ec5bdb3
Chetty, Alisha
15f186e0-b5d0-48f7-b3c3-876b43b95d64
Smith, Katherine
9093b9f6-16a3-4d4d-8361-a8be37e4fe2d
Darby, Matthew
c891f9aa-aa3f-46cf-95fb-9452244f95aa
Roberts, Luke
f4bf25e5-b7e9-402a-a26b-14adc38d51da
Mackay, Rosie-Marie
19cf1b92-c65d-4baa-a165-ab630bf77ec3
Whitwell, Harry J.
26aa6224-2eac-4932-9780-3f9e9c165d23
Timms, John F.
ce6b31c4-143d-49f5-a8bc-05d2633c8400
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Selkirk, Murray E.
08a84a3d-778e-49eb-8afc-9e65bda5d311
Brombacher, Frank
222b7d91-a402-4cf9-b56f-97bdfdbe1a2a
Clark, Howard William
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Horsnell, William G.C.
b490aa17-3f96-49d3-8b02-821bf14e2537
Thawer, Sumaiyya
c595802c-3ced-4578-a07e-9453233a6ba6
Auret, Jennifer
255c6da8-b1ad-4ccb-87c8-cfe99a142a91
Schnoeller, Corinna
f6a29996-68f4-4798-94e2-c5d15ec5bdb3
Chetty, Alisha
15f186e0-b5d0-48f7-b3c3-876b43b95d64
Smith, Katherine
9093b9f6-16a3-4d4d-8361-a8be37e4fe2d
Darby, Matthew
c891f9aa-aa3f-46cf-95fb-9452244f95aa
Roberts, Luke
f4bf25e5-b7e9-402a-a26b-14adc38d51da
Mackay, Rosie-Marie
19cf1b92-c65d-4baa-a165-ab630bf77ec3
Whitwell, Harry J.
26aa6224-2eac-4932-9780-3f9e9c165d23
Timms, John F.
ce6b31c4-143d-49f5-a8bc-05d2633c8400
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Selkirk, Murray E.
08a84a3d-778e-49eb-8afc-9e65bda5d311
Brombacher, Frank
222b7d91-a402-4cf9-b56f-97bdfdbe1a2a
Clark, Howard William
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Horsnell, William G.C.
b490aa17-3f96-49d3-8b02-821bf14e2537

Thawer, Sumaiyya, Auret, Jennifer, Schnoeller, Corinna, Chetty, Alisha, Smith, Katherine, Darby, Matthew, Roberts, Luke, Mackay, Rosie-Marie, Whitwell, Harry J., Timms, John F., Madsen, Jens, Selkirk, Murray E., Brombacher, Frank, Clark, Howard William and Horsnell, William G.C. (2016) Surfactant Protein-D is essential for immunity to helminth infection. PLoS Pathogens, 12 (2), 1-18. (doi:10.1371/journal.ppat.1005461). (PMID:26900854)

Record type: Article

Abstract

Pulmonary epithelial cell responses can enhance type 2 immunity and contribute to control of nematode infections. An important epithelial product is the collectin Surfactant Protein D (SP-D). We found that SP-D concentrations increased in the lung following Nippostrongylus brasiliensis infection; this increase was dependent on key components of the type 2 immune response. We carried out loss and gain of function studies of SP-D to establish if SP-D was required for optimal immunity to the parasite. N. brasiliensis infection of SP-D-/- mice resulted in profound impairment of host innate immunity and ability to resolve infection. Raising pulmonary SP-D levels prior to infection enhanced parasite expulsion and type 2 immune responses, including increased numbers of IL-13 producing type 2 innate lymphoid cells (ILC2), elevated expression of markers of alternative activation by alveolar macrophages (alvM) and increased production of the type 2 cytokines IL-4 and IL-13. Adoptive transfer of alvM from SP-D-treated parasite infected mice into naïve recipients enhanced immunity to N. brasiliensis. Protection was associated with selective binding by the SP-D carbohydrate recognition domain (CRD) to L4 parasites to enhance their killing by alvM. These findings are the first demonstration that the collectin SP-D is an essential component of host innate immunity to helminths.

Text
THAWER - PLoS Pathogens - SPD is essential for immunity in helminth infection.pdf - Version of Record
Available under License Creative Commons Attribution.
Download (2MB)

More information

Accepted/In Press date: 28 January 2016
Published date: 22 February 2016
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 395458
URI: https://eprints.soton.ac.uk/id/eprint/395458
ISSN: 1553-7366
PURE UUID: 96f8317f-d95f-4b9b-8175-40b7c8de42e6
ORCID for Rosie-Marie Mackay: ORCID iD orcid.org/0000-0002-9493-9654
ORCID for Jens Madsen: ORCID iD orcid.org/0000-0003-1664-7645

Catalogue record

Date deposited: 31 May 2016 10:06
Last modified: 10 Dec 2019 01:42

Export record

Altmetrics

Contributors

Author: Sumaiyya Thawer
Author: Jennifer Auret
Author: Corinna Schnoeller
Author: Alisha Chetty
Author: Katherine Smith
Author: Matthew Darby
Author: Luke Roberts
Author: Rosie-Marie Mackay ORCID iD
Author: Harry J. Whitwell
Author: John F. Timms
Author: Jens Madsen ORCID iD
Author: Murray E. Selkirk
Author: Frank Brombacher
Author: William G.C. Horsnell

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×