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NMR of 133Cs+ in stretched hydrogels: one-dimensional, z- and NOESY spectra, and probing the ion’s environment in erythrocytes

NMR of 133Cs+ in stretched hydrogels: one-dimensional, z- and NOESY spectra, and probing the ion’s environment in erythrocytes
NMR of 133Cs+ in stretched hydrogels: one-dimensional, z- and NOESY spectra, and probing the ion’s environment in erythrocytes
(133)Cs nuclear magnetic resonance (NMR) spectroscopy was conducted on (133)Cs(+) in gelatin hydrogels that were either relaxed or stretched. Stretching generated a septet from this spin-7/2 nucleus, and its nuclear magnetic relaxation was studied via z-spectra, and two-dimensional nuclear Overhauser (NOESY) spectroscopy. Various spectral features were well simulated by using Mathematica and the software package SpinDynamica. Spectra of CsCl in suspensions of human erythrocytes embedded in gelatin gel showed separation of the resonances from the cation inside and outside the cells. Upon stretching the sample, the extracellular (133)Cs(+) signal split into a septet, while the intracellular peak was unchanged, revealing different alignment/ordering properties of the environment inside and around the cells. Differential interference contrast light microscopy confirmed that the cells were stretched when the overall sample was elongated. Analysis of the various spectral features of (133)Cs(+) reported here opens up applications of this K(+) congener for studies of cation-handling by metabolically-active cells and tissues in aligned states.
110-120
Kuchel, Philip W.
62f434b1-d370-4552-85f4-05b76c625945
Shishmarev, Dmitry
11bac3f9-42c3-42ba-b3d1-e5fc06365580
Puckeridge, Max
f5af8c9e-43a3-4203-82f2-ba2e5d3f3308
Levitt, Malcolm
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Naumann, Christoph
2ebe1944-96a5-4489-9010-a7d9b6539b3e
Chapman, Bogdan E.
1442dfa9-6e1b-4348-8e2c-44f0f105ddd5
Kuchel, Philip W.
62f434b1-d370-4552-85f4-05b76c625945
Shishmarev, Dmitry
11bac3f9-42c3-42ba-b3d1-e5fc06365580
Puckeridge, Max
f5af8c9e-43a3-4203-82f2-ba2e5d3f3308
Levitt, Malcolm
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Naumann, Christoph
2ebe1944-96a5-4489-9010-a7d9b6539b3e
Chapman, Bogdan E.
1442dfa9-6e1b-4348-8e2c-44f0f105ddd5

Kuchel, Philip W., Shishmarev, Dmitry, Puckeridge, Max, Levitt, Malcolm, Naumann, Christoph and Chapman, Bogdan E. (2015) NMR of 133Cs+ in stretched hydrogels: one-dimensional, z- and NOESY spectra, and probing the ion’s environment in erythrocytes. Journal of Magnetic Resonance, 261, 110-120. (PMID:26561738)

Record type: Article

Abstract

(133)Cs nuclear magnetic resonance (NMR) spectroscopy was conducted on (133)Cs(+) in gelatin hydrogels that were either relaxed or stretched. Stretching generated a septet from this spin-7/2 nucleus, and its nuclear magnetic relaxation was studied via z-spectra, and two-dimensional nuclear Overhauser (NOESY) spectroscopy. Various spectral features were well simulated by using Mathematica and the software package SpinDynamica. Spectra of CsCl in suspensions of human erythrocytes embedded in gelatin gel showed separation of the resonances from the cation inside and outside the cells. Upon stretching the sample, the extracellular (133)Cs(+) signal split into a septet, while the intracellular peak was unchanged, revealing different alignment/ordering properties of the environment inside and around the cells. Differential interference contrast light microscopy confirmed that the cells were stretched when the overall sample was elongated. Analysis of the various spectral features of (133)Cs(+) reported here opens up applications of this K(+) congener for studies of cation-handling by metabolically-active cells and tissues in aligned states.

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More information

e-pub ahead of print date: 27 October 2015
Published date: December 2015
Organisations: Magnetic Resonance

Identifiers

Local EPrints ID: 395476
URI: https://eprints.soton.ac.uk/id/eprint/395476
PURE UUID: be602369-6d13-4765-aab0-7b6ad3fa7763
ORCID for Malcolm Levitt: ORCID iD orcid.org/0000-0001-9878-1180

Catalogue record

Date deposited: 31 May 2016 10:43
Last modified: 14 Jul 2018 00:34

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