An accurate in vitro model of the E. coli envelope
An accurate in vitro model of the E. coli envelope
Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir-Blodgett and Langmuir-Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.
11952-11955
Clifton, Luke A.
025a4531-f08a-4d2c-87c5-24d17f8afda4
Holt, Stephen A.
9f0ab248-5e44-4a06-8cc2-b59113293831
Hughes, Arwel V.
2dd18a2f-5724-44a3-ba82-951133253cb2
Daulton, Emma L.
784c2023-fa32-4609-a6f1-7f965518aa42
Arunmanee, Wanatchaporn
5d30b6aa-532a-468d-97a1-22e2e88e2804
Heinrich, Frank
ba765a1d-c071-4ae9-b532-b8dcc0fc2966
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394
Jefferies, Damien
8df97e21-8df6-4571-bfbe-3edc41e16967
Charlton, Timothy R.
f24bdc97-17e7-402c-944a-1a6e8f799945
Webster, John R.P.
24ff8ad7-92bb-492d-a4c7-86e8cdd5c59f
Kinane, Christian J.
97cb4260-c5cc-49af-8c12-99d138ff1ca9
Lakey, Jeremy H.
6f7be39e-7944-421d-b638-60d245829f10
29 September 2015
Clifton, Luke A.
025a4531-f08a-4d2c-87c5-24d17f8afda4
Holt, Stephen A.
9f0ab248-5e44-4a06-8cc2-b59113293831
Hughes, Arwel V.
2dd18a2f-5724-44a3-ba82-951133253cb2
Daulton, Emma L.
784c2023-fa32-4609-a6f1-7f965518aa42
Arunmanee, Wanatchaporn
5d30b6aa-532a-468d-97a1-22e2e88e2804
Heinrich, Frank
ba765a1d-c071-4ae9-b532-b8dcc0fc2966
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394
Jefferies, Damien
8df97e21-8df6-4571-bfbe-3edc41e16967
Charlton, Timothy R.
f24bdc97-17e7-402c-944a-1a6e8f799945
Webster, John R.P.
24ff8ad7-92bb-492d-a4c7-86e8cdd5c59f
Kinane, Christian J.
97cb4260-c5cc-49af-8c12-99d138ff1ca9
Lakey, Jeremy H.
6f7be39e-7944-421d-b638-60d245829f10
Clifton, Luke A., Holt, Stephen A., Hughes, Arwel V., Daulton, Emma L., Arunmanee, Wanatchaporn, Heinrich, Frank, Khalid, Syma, Jefferies, Damien, Charlton, Timothy R., Webster, John R.P., Kinane, Christian J. and Lakey, Jeremy H.
(2015)
An accurate in vitro model of the E. coli envelope.
Angewandte Chemie International Edition, 54 (41), .
(doi:10.1002/anie.201504287).
(PMID:26331292)
Abstract
Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir-Blodgett and Langmuir-Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.
Text
11952_ftp.pdf
- Version of Record
More information
e-pub ahead of print date: 1 September 2015
Published date: 29 September 2015
Organisations:
Computational Systems Chemistry
Identifiers
Local EPrints ID: 395514
URI: http://eprints.soton.ac.uk/id/eprint/395514
ISSN: 1433-7851
PURE UUID: 46142fb7-1518-4f02-bf67-6acd636ca1c1
Catalogue record
Date deposited: 31 May 2016 14:43
Last modified: 15 Mar 2024 03:29
Export record
Altmetrics
Contributors
Author:
Luke A. Clifton
Author:
Stephen A. Holt
Author:
Arwel V. Hughes
Author:
Emma L. Daulton
Author:
Wanatchaporn Arunmanee
Author:
Frank Heinrich
Author:
Syma Khalid
Author:
Damien Jefferies
Author:
Timothy R. Charlton
Author:
John R.P. Webster
Author:
Christian J. Kinane
Author:
Jeremy H. Lakey
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics