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High accuracy particle analysis using sheathless microfluidic impedance cytometry

High accuracy particle analysis using sheathless microfluidic impedance cytometry
High accuracy particle analysis using sheathless microfluidic impedance cytometry
This paper describes a new design of microfluidic impedance cytometer enabling accurate characterization of particles without the need for focusing. The approach uses multiple pairs of electrodes to measure the transit time of particles through the device in two simultaneous different current measurements, a transverse (top to bottom) current and an oblique current. This gives a new metric that can be used to estimate the vertical position of the particle trajectory through the microchannel. This parameter effectively compensates for the non-uniform electric field in the channel that is an unavoidable consequence of the use of planar parallel facing electrodes. The new technique is explained and validated using numerical modelling. Impedance data for 5, 6 and 7 µm particles are collected and compared with simulations. The method gives excellent coefficient of variation in (electrical) radius of particles of 1% for a sheathless configuration.
1473-0197
1-8
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Caselli, Frederica
141b5a89-9e4d-47ac-a015-e65534f8d71b
Bisenga, Paolo
69b30eef-6e8c-4d80-a90c-73572e4296d1
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Caselli, Frederica
141b5a89-9e4d-47ac-a015-e65534f8d71b
Bisenga, Paolo
69b30eef-6e8c-4d80-a90c-73572e4296d1
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Spencer, Daniel, Caselli, Frederica, Bisenga, Paolo and Morgan, Hywel (2016) High accuracy particle analysis using sheathless microfluidic impedance cytometry. Lab on a Chip, 1-8. (doi:10.1039/C6LC00339G).

Record type: Article

Abstract

This paper describes a new design of microfluidic impedance cytometer enabling accurate characterization of particles without the need for focusing. The approach uses multiple pairs of electrodes to measure the transit time of particles through the device in two simultaneous different current measurements, a transverse (top to bottom) current and an oblique current. This gives a new metric that can be used to estimate the vertical position of the particle trajectory through the microchannel. This parameter effectively compensates for the non-uniform electric field in the channel that is an unavoidable consequence of the use of planar parallel facing electrodes. The new technique is explained and validated using numerical modelling. Impedance data for 5, 6 and 7 µm particles are collected and compared with simulations. The method gives excellent coefficient of variation in (electrical) radius of particles of 1% for a sheathless configuration.

Other C6LC00339G_page=search - Accepted Manuscript
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More information

Accepted/In Press date: 20 May 2016
e-pub ahead of print date: 20 May 2016
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 395519
URI: https://eprints.soton.ac.uk/id/eprint/395519
ISSN: 1473-0197
PURE UUID: d1b1c83b-08c7-4959-982d-3edbb3d641f6
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 31 May 2016 15:18
Last modified: 04 Jul 2018 04:01

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