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Novel insight into the immunological basis of chronic granulomatous invasive fungal rhinosinusitis

Novel insight into the immunological basis of chronic granulomatous invasive fungal rhinosinusitis
Novel insight into the immunological basis of chronic granulomatous invasive fungal rhinosinusitis
Background - Chronic granulomatous invasive fungal rhinosinusitis (CGIFRS) is a rare disease. The underlying immune responses that drive the development of CGIFRS, as opposed to successful pathogen clearance and controlled inflammation, are not currently known.

Objective - This study aimed to characterise the immune responses associated with CGIFRS

Methodology - In addition to a battery of basic investigations, more in depth immunological testing involving ex-vivo whole blood stimulation with the polyclonal T cell mitogen phytohaemaglutinin and fungal antigens with interleukin (IL)-12, was undertaken to investigate cell-mediated immune responses associated with CGIFRS.

Results - Ex-vivo whole blood stimulation with the polyclonal T cell mitogen phytohaemaglutinin and fungal antigens with IL-12 identified reduced interferon-gamma (IFN-?) and increased IL-17A levels within the supernatant, suggesting increased in-vivo T helper (Th)17 responses and impaired Th1 responses compared to healthy control.

Conclusion - These findings suggest that the development of CGIFRS may be associated with an abnormally exaggerated host Th17 responses causing failure to clear the fungal pathogen with refractory fungal infection of mucosal membranes, resulting in chronic tissue inflammation.
2152-6575
e102-e106
Rae, W.
f929f61b-adc1-4438-95b4-30777cd609cf
Doffinger, R.
7057457b-d2c9-471e-91f2-138dca9beac6
Shelton, F.
afa14968-01fb-494b-9dac-8b0de5197d5b
Sproson, E.
35ea1b78-11ce-40c4-babb-36faea1a25b1
Ismail-Koch, H.
c9075c56-cd32-4075-8ab6-fbe96859b688
Lund, V.
19b0121c-1518-4cd8-8c2b-be8557320b0f
Harries, P.
faf478fc-e9e2-47a0-92aa-8d3590fdd179
Eren, E.
ac449fc8-4ae2-4efd-ad91-9dcea3f355e2
Salib, R.
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc
Rae, W.
f929f61b-adc1-4438-95b4-30777cd609cf
Doffinger, R.
7057457b-d2c9-471e-91f2-138dca9beac6
Shelton, F.
afa14968-01fb-494b-9dac-8b0de5197d5b
Sproson, E.
35ea1b78-11ce-40c4-babb-36faea1a25b1
Ismail-Koch, H.
c9075c56-cd32-4075-8ab6-fbe96859b688
Lund, V.
19b0121c-1518-4cd8-8c2b-be8557320b0f
Harries, P.
faf478fc-e9e2-47a0-92aa-8d3590fdd179
Eren, E.
ac449fc8-4ae2-4efd-ad91-9dcea3f355e2
Salib, R.
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc

Rae, W., Doffinger, R. and Shelton, F. et al. (2016) Novel insight into the immunological basis of chronic granulomatous invasive fungal rhinosinusitis. Allergy & Rhinology, 7 (2), e102-e106. (doi:10.2500/ar.2016.7.0162).

Record type: Article

Abstract

Background - Chronic granulomatous invasive fungal rhinosinusitis (CGIFRS) is a rare disease. The underlying immune responses that drive the development of CGIFRS, as opposed to successful pathogen clearance and controlled inflammation, are not currently known.

Objective - This study aimed to characterise the immune responses associated with CGIFRS

Methodology - In addition to a battery of basic investigations, more in depth immunological testing involving ex-vivo whole blood stimulation with the polyclonal T cell mitogen phytohaemaglutinin and fungal antigens with interleukin (IL)-12, was undertaken to investigate cell-mediated immune responses associated with CGIFRS.

Results - Ex-vivo whole blood stimulation with the polyclonal T cell mitogen phytohaemaglutinin and fungal antigens with IL-12 identified reduced interferon-gamma (IFN-?) and increased IL-17A levels within the supernatant, suggesting increased in-vivo T helper (Th)17 responses and impaired Th1 responses compared to healthy control.

Conclusion - These findings suggest that the development of CGIFRS may be associated with an abnormally exaggerated host Th17 responses causing failure to clear the fungal pathogen with refractory fungal infection of mucosal membranes, resulting in chronic tissue inflammation.

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Accepted/In Press date: 10 May 2016
Published date: 2016
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 396004
URI: https://eprints.soton.ac.uk/id/eprint/396004
ISSN: 2152-6575
PURE UUID: 79397f66-ff29-4fda-b374-496ac97fb278
ORCID for R. Salib: ORCID iD orcid.org/0000-0002-6753-7844

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Date deposited: 02 Jun 2016 10:45
Last modified: 20 Aug 2019 04:02

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Contributors

Author: W. Rae
Author: R. Doffinger
Author: F. Shelton
Author: E. Sproson
Author: H. Ismail-Koch
Author: V. Lund
Author: P. Harries
Author: E. Eren
Author: R. Salib ORCID iD

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