The "Antimicrobial_killing_depth_analysis.opj" is an Origin (http://www.originlab.com/) spreadsheet showing the calculations of the relative depth of killing as taken from the confocal stacks of live (green) and dead (red) cells at different depths in the biofilm. The "Beads_relative_depth_Analysis.opj" is an is an Origin (http://www.originlab.com/) spreadsheet showing the calculations of the relative penetration depth of fluorescent beads from either diffusion, shaking or after shooting with the Sonicare AirFloss. The data were extracted from confocal stacks of the biofilm.