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Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP–peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP–peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP-based formulations suitable for the selective modulation of angiogenesis, which is of paramount importance in applications where promotion of vascular growth is desirable (eg, wound healing) or ought to be contravened, as in cancer development.
angiogenesis activators, antiangiogenic, CAM assay, gold nanoparticles, peptide-coated gold nanoparticles, vascular development
1176-9114
2633-2639
Roma-Rodrigues, Catarina
50cb6890-7e99-490d-b295-c4e44aa5ab93
Heuer-Jungemann, Amelie
19d5a272-0f65-4679-b62b-263fa1806230
Fernandes, Alexandra
2c686d7b-dd0b-4eb0-8731-6bff67106966
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Baptista, Pedro
d2fbef22-44c4-4691-a54c-29415e7e5950
Roma-Rodrigues, Catarina
50cb6890-7e99-490d-b295-c4e44aa5ab93
Heuer-Jungemann, Amelie
19d5a272-0f65-4679-b62b-263fa1806230
Fernandes, Alexandra
2c686d7b-dd0b-4eb0-8731-6bff67106966
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Baptista, Pedro
d2fbef22-44c4-4691-a54c-29415e7e5950

Roma-Rodrigues, Catarina, Heuer-Jungemann, Amelie, Fernandes, Alexandra, Kanaras, Antonios and Baptista, Pedro (2016) Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo. International Journal of Nanomedicine, 2016 (11), 2633-2639. (doi:10.2147/IJN.S108661).

Record type: Article

Abstract

In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP–peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP–peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP-based formulations suitable for the selective modulation of angiogenesis, which is of paramount importance in applications where promotion of vascular growth is desirable (eg, wound healing) or ought to be contravened, as in cancer development.

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2016-International Journal of Nanomedicine.pdf - Version of Record
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More information

Accepted/In Press date: 14 April 2016
e-pub ahead of print date: 7 June 2016
Keywords: angiogenesis activators, antiangiogenic, CAM assay, gold nanoparticles, peptide-coated gold nanoparticles, vascular development
Organisations: Quantum, Light & Matter Group

Identifiers

Local EPrints ID: 396689
URI: https://eprints.soton.ac.uk/id/eprint/396689
ISSN: 1176-9114
PURE UUID: 38f486c5-a5d7-441a-8158-c698091b3f16
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 10 Jun 2016 13:26
Last modified: 06 Jun 2018 12:37

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