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Ion channels made from a single membrane-spanning DNA duplex

Ion channels made from a single membrane-spanning DNA duplex
Ion channels made from a single membrane-spanning DNA duplex
Because of their hollow interior, transmembrane channels are capable of opening up pathways for ions across lipid membranes of living cells. Here, we demonstrate ion conduction induced by a single DNA duplex that lacks a hollow central channel. Decorated with six porpyrin-tags, our duplex is designed to span lipid membranes. Combining electrophysiology measurements with all-atom molecular dynamics simulations, we elucidate the microscopic conductance pathway. Ions flow at the DNA–lipid interface as the lipid head groups tilt toward the amphiphilic duplex forming a toroidal pore filled with water and ions. Ionic current traces produced by the DNA-lipid channel show well-defined insertion steps, closures, and gating similar to those observed for traditional protein channels or synthetic pores. Ionic conductances obtained through simulations and experiments are in excellent quantitative agreement. The conductance mechanism realized here with the smallest possible DNA-based ion channel offers a route to design a new class of synthetic ion channels with maximum simplicity.
1530-6984
4665-4669
Göpfrich, Kerstin
c3fab0e7-5324-490e-b748-b9f8409a51ef
Li, Chen-Yu
625a8707-6dc4-4cb5-a7b8-7bc0d5353d56
Mames, Iwona
c7177d38-bda3-44d2-9140-9cfb9624ca49
Bhamidimarri, Satya Prathyusha
743238c8-95bc-4b19-93c5-76117dc36b44
Ricci, Maria
69c4d009-b094-439e-89ed-609576260262
Yoo, Jejoong
e9ff320b-bf18-46bb-b847-d2a226ef14a2
Mames, Adam
608b81ab-87c3-4c8c-863a-54b3d21ae9d3
Ohmann, Alexander
381df4a4-812e-4c1c-a602-00cdfa0762df
Winterhalter, Mathias
e1b3741c-2088-4cdd-b0b9-5a3a1e49a190
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Aksimentiev, Aleksei
9527b04a-ad60-4569-b2bf-d15bb0ee4f7b
Keyser, Ulrich F
2bf2fc35-efb2-4cc3-b5a2-817e55fe9115
Göpfrich, Kerstin
c3fab0e7-5324-490e-b748-b9f8409a51ef
Li, Chen-Yu
625a8707-6dc4-4cb5-a7b8-7bc0d5353d56
Mames, Iwona
c7177d38-bda3-44d2-9140-9cfb9624ca49
Bhamidimarri, Satya Prathyusha
743238c8-95bc-4b19-93c5-76117dc36b44
Ricci, Maria
69c4d009-b094-439e-89ed-609576260262
Yoo, Jejoong
e9ff320b-bf18-46bb-b847-d2a226ef14a2
Mames, Adam
608b81ab-87c3-4c8c-863a-54b3d21ae9d3
Ohmann, Alexander
381df4a4-812e-4c1c-a602-00cdfa0762df
Winterhalter, Mathias
e1b3741c-2088-4cdd-b0b9-5a3a1e49a190
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Aksimentiev, Aleksei
9527b04a-ad60-4569-b2bf-d15bb0ee4f7b
Keyser, Ulrich F
2bf2fc35-efb2-4cc3-b5a2-817e55fe9115

Göpfrich, Kerstin, Li, Chen-Yu, Mames, Iwona, Bhamidimarri, Satya Prathyusha, Ricci, Maria, Yoo, Jejoong, Mames, Adam, Ohmann, Alexander, Winterhalter, Mathias, Stulz, Eugen, Aksimentiev, Aleksei and Keyser, Ulrich F (2016) Ion channels made from a single membrane-spanning DNA duplex. Nano Letters, 16 (7), 4665-4669. (doi:10.1021/acs.nanolett.6b02039).

Record type: Article

Abstract

Because of their hollow interior, transmembrane channels are capable of opening up pathways for ions across lipid membranes of living cells. Here, we demonstrate ion conduction induced by a single DNA duplex that lacks a hollow central channel. Decorated with six porpyrin-tags, our duplex is designed to span lipid membranes. Combining electrophysiology measurements with all-atom molecular dynamics simulations, we elucidate the microscopic conductance pathway. Ions flow at the DNA–lipid interface as the lipid head groups tilt toward the amphiphilic duplex forming a toroidal pore filled with water and ions. Ionic current traces produced by the DNA-lipid channel show well-defined insertion steps, closures, and gating similar to those observed for traditional protein channels or synthetic pores. Ionic conductances obtained through simulations and experiments are in excellent quantitative agreement. The conductance mechanism realized here with the smallest possible DNA-based ion channel offers a route to design a new class of synthetic ion channels with maximum simplicity.

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Accepted/In Press date: 21 June 2016
e-pub ahead of print date: 29 June 2016
Published date: 13 July 2016
Organisations: Chemical Biology Group

Identifiers

Local EPrints ID: 397209
URI: http://eprints.soton.ac.uk/id/eprint/397209
ISSN: 1530-6984
PURE UUID: e68319fa-b80f-4ab1-abed-1abc4b3d289e
ORCID for Eugen Stulz: ORCID iD orcid.org/0000-0002-5302-2276

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Date deposited: 24 Jun 2016 09:27
Last modified: 07 Oct 2020 05:12

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Contributors

Author: Kerstin Göpfrich
Author: Chen-Yu Li
Author: Iwona Mames
Author: Satya Prathyusha Bhamidimarri
Author: Maria Ricci
Author: Jejoong Yoo
Author: Adam Mames
Author: Alexander Ohmann
Author: Mathias Winterhalter
Author: Eugen Stulz ORCID iD
Author: Aleksei Aksimentiev
Author: Ulrich F Keyser

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