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Squamous metaplasia is increased in the bronchial epithelium of smokers with chronic obstructive pulmonary disease

Squamous metaplasia is increased in the bronchial epithelium of smokers with chronic obstructive pulmonary disease
Squamous metaplasia is increased in the bronchial epithelium of smokers with chronic obstructive pulmonary disease
Aims

To quantify the extent of squamous metaplasia in bronchial biopsies and relate it to the presence of chronic obstructive pulmonary disease (COPD), a smoking-related pathology.

Methods

Bronchial biopsies (n = 15 in each group) from smokers with COPD GOLD stage1 and GOLD stage2, smokers without COPD and healthy non-smokers were stained immunohistochemically with a panel of antibodies that facilitated the identification of pseudostratified epithelium and distinction of squamous metaplasia and squamous epithelium from tangentially cut epithelium. The percentage length of each of these epithelial phenotypes was measured as a percent of total epithelial length using computerised image analysis. Sections were also stained for carcinoembryonic antigen and p53, early markers of carcinogenesis, and Ki67, and the percentage epithelial expression measured.

Results

The extent of squamous metaplasia was significantly increased in both COPD1 and COPD2 compared to healthy smokers and healthy non-smokers. The amount of fully differentiated squamous epithelium was also increased in COPD1 and COPD2 compared to healthy non-smokers, as was the expression of carcinoembryonic antigen. These features correlated with one other.

Conclusion

In subjects with COPD there is a loss of pseudostratified epithelium accompanied by an increase in squamous metaplasia with transition into a fully squamous epithelium and expression of early markers of carcinogenesis.
1932-6203
1-11
Rigden, Helen M.
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Alias, Ahmad
ff37ac72-f27d-4c86-8e03-912422d264fc
Havelock, Tom
8f9aa3be-a768-4a7b-bcd4-79656e2b188c
O'Donnell, Rory
1caff59c-ac09-49cb-8d03-ca85be62613e
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Rigden, Helen M.
3f3220f6-7615-41f9-81b5-04f20601d49b
Alias, Ahmad
ff37ac72-f27d-4c86-8e03-912422d264fc
Havelock, Tom
8f9aa3be-a768-4a7b-bcd4-79656e2b188c
O'Donnell, Rory
1caff59c-ac09-49cb-8d03-ca85be62613e
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8

Rigden, Helen M., Alias, Ahmad, Havelock, Tom, O'Donnell, Rory, Djukanovic, Ratko, Davies, Donna E. and Wilson, Susan J. (2016) Squamous metaplasia is increased in the bronchial epithelium of smokers with chronic obstructive pulmonary disease. PLoS ONE, 11 (5), 1-11. (doi:10.1371/journal.pone.0156009). (PMID:27228128)

Record type: Article

Abstract

Aims

To quantify the extent of squamous metaplasia in bronchial biopsies and relate it to the presence of chronic obstructive pulmonary disease (COPD), a smoking-related pathology.

Methods

Bronchial biopsies (n = 15 in each group) from smokers with COPD GOLD stage1 and GOLD stage2, smokers without COPD and healthy non-smokers were stained immunohistochemically with a panel of antibodies that facilitated the identification of pseudostratified epithelium and distinction of squamous metaplasia and squamous epithelium from tangentially cut epithelium. The percentage length of each of these epithelial phenotypes was measured as a percent of total epithelial length using computerised image analysis. Sections were also stained for carcinoembryonic antigen and p53, early markers of carcinogenesis, and Ki67, and the percentage epithelial expression measured.

Results

The extent of squamous metaplasia was significantly increased in both COPD1 and COPD2 compared to healthy smokers and healthy non-smokers. The amount of fully differentiated squamous epithelium was also increased in COPD1 and COPD2 compared to healthy non-smokers, as was the expression of carcinoembryonic antigen. These features correlated with one other.

Conclusion

In subjects with COPD there is a loss of pseudostratified epithelium accompanied by an increase in squamous metaplasia with transition into a fully squamous epithelium and expression of early markers of carcinogenesis.

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More information

Accepted/In Press date: 6 May 2016
e-pub ahead of print date: 26 May 2016
Published date: 26 May 2016
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 397524
URI: https://eprints.soton.ac.uk/id/eprint/397524
ISSN: 1932-6203
PURE UUID: acc7d510-4f38-4229-a412-8fc10aeb9966
ORCID for Ratko Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991

Catalogue record

Date deposited: 30 Jun 2016 11:46
Last modified: 10 Dec 2019 01:59

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