Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.
Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.
Human bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors — TGF? receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3? inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Pluritest
128-132
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Megges, M.
724e45bf-6f8d-4ae9-8bc7-da0e14bbee18
Adjaye, J.
8d726041-3cd1-436e-8bca-730c523842d4
28 December 2015
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Megges, M.
724e45bf-6f8d-4ae9-8bc7-da0e14bbee18
Adjaye, J.
8d726041-3cd1-436e-8bca-730c523842d4
Oreffo, Richard, Megges, M. and Adjaye, J.
(2015)
Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.
Stem Cell Research, 16 (1), .
(doi:10.1016/j.scr.2015.12.013).
(PMID:27345797)
Abstract
Human bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors — TGF? receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3? inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Pluritest
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Published date: 28 December 2015
Organisations:
Human Development & Health
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Local EPrints ID: 397913
URI: http://eprints.soton.ac.uk/id/eprint/397913
ISSN: 1873-5061
PURE UUID: beed0266-0f36-4ca6-8d5f-5e1089a33b0f
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Date deposited: 11 Jul 2016 10:29
Last modified: 15 Mar 2024 03:04
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Author:
M. Megges
Author:
J. Adjaye
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