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Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation

Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation
Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation
Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study, we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation, hence better informing biological mechanisms of stem cell growth. Tools that are able to investigate the stem cell phenotype are important. While large phenotypical differences, such as the difference between an adipocyte and an osteoblast, are now better understood, the far more subtle differences between fibroblasts and MSCs are much harder to dissect. The development of technologies able to dynamically navigate small differences in adhesion are critical in the race to provide regenerative strategies using stem cells
1936-0851
6667-6679
Roberts, Jemma N.
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Sahoo, Jugal Kishore
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McNamara, Laura E.
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Burgess, Karl V.
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Yang, Jingli
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Alakpa, Enateri V.
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Anderson, Hilary J.
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Hay, Jake
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Turner, Lesley-Anne
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Yarwood, Stephen J.
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Zelzer, Mischa
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Oreffo, Richard
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Ulijn, Rein V.
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Dalby, Matthew J.
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Roberts, Jemma N.
dab50844-4015-4a42-9876-932c8a94c875
Sahoo, Jugal Kishore
f7cb77b5-8f63-4f79-8666-9b2b4448bf2a
McNamara, Laura E.
c5efd583-f6a3-4907-b357-1ccf4dba31cb
Burgess, Karl V.
92de78f3-7b0d-4a93-9672-25bdc66cbb2b
Yang, Jingli
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Alakpa, Enateri V.
1ea4c26a-fbf2-46b4-ae76-35a181ec96df
Anderson, Hilary J.
6b9fe341-4897-44a9-b87b-02b3a3060009
Hay, Jake
d95b4068-71c9-44c8-9b43-00661c11f11e
Turner, Lesley-Anne
ea8359b4-809a-48fc-8858-a309eda31a50
Yarwood, Stephen J.
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Zelzer, Mischa
ae3aea77-87e0-4cb0-8a62-3aa654fdfddf
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Ulijn, Rein V.
0bcc5fd0-94e4-4b62-8f32-6860d968c321
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc

Roberts, Jemma N., Sahoo, Jugal Kishore, McNamara, Laura E., Burgess, Karl V., Yang, Jingli, Alakpa, Enateri V., Anderson, Hilary J., Hay, Jake, Turner, Lesley-Anne, Yarwood, Stephen J., Zelzer, Mischa, Oreffo, Richard, Ulijn, Rein V. and Dalby, Matthew J. (2016) Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation. ACS Nano, 10 (7), 6667-6679. (doi:10.1021/acsnano.6b01765). (PMID:27322014)

Record type: Article

Abstract

Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study, we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation, hence better informing biological mechanisms of stem cell growth. Tools that are able to investigate the stem cell phenotype are important. While large phenotypical differences, such as the difference between an adipocyte and an osteoblast, are now better understood, the far more subtle differences between fibroblasts and MSCs are much harder to dissect. The development of technologies able to dynamically navigate small differences in adhesion are critical in the race to provide regenerative strategies using stem cells

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More information

Accepted/In Press date: 20 June 2016
e-pub ahead of print date: 27 June 2016
Published date: July 2016
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 397916
URI: http://eprints.soton.ac.uk/id/eprint/397916
ISSN: 1936-0851
PURE UUID: 256e6d72-5ff4-46c8-b9c9-59aff764cbfe
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 11 Jul 2016 10:59
Last modified: 15 Mar 2024 03:04

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Contributors

Author: Jemma N. Roberts
Author: Jugal Kishore Sahoo
Author: Laura E. McNamara
Author: Karl V. Burgess
Author: Jingli Yang
Author: Enateri V. Alakpa
Author: Hilary J. Anderson
Author: Jake Hay
Author: Lesley-Anne Turner
Author: Stephen J. Yarwood
Author: Mischa Zelzer
Author: Richard Oreffo ORCID iD
Author: Rein V. Ulijn
Author: Matthew J. Dalby

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