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Tapping the unused potential of photosynthesis with a heterologous electron sink

Tapping the unused potential of photosynthesis with a heterologous electron sink
Tapping the unused potential of photosynthesis with a heterologous electron sink
Increasing the efficiency of the conversion of light energy to products by photosynthesis represents a grand challenge in biotechnology. Photosynthesis is limited by the carbon-fixing enzyme Rubisco resulting in much of the absorbed energy being wasted as heat, fluorescence or lost as excess reductant via alternative electron dissipation pathways. To harness this wasted reductant, we engineered the model cyanobacterium Synechococcus PCC 7002 to express the mammalian cytochrome P450 CYP1A1 to serve as an artificial electron sink for excess electrons derived from light-catalysed water-splitting. This improved photosynthetic efficiency by increasing the maximum rate of photosynthetic electron flow by 31.3%. A simple fluorescent assay for CYP1A1 activity demonstrated that the P450 was functional in the absence of its native reductase, that activity was light-dependent and scaled with irradiance. We show for the first time in live cells that photosynthetic reductant can be redirected to power a heterologous cytochrome P450. Furthermore, PCC 7002 expressing CYP1A1 degraded the herbicide atrazine, which is a widespread environmental pollutant.
1369–1375
Berepiki, Adokiye
1be9b7f8-011f-454c-bc9e-244b2c07799a
Hitchcock, Andrew
aeca86f3-e8cf-47d1-9338-e82630fb868e
Moore, Christopher Mark
7ec80b7b-bedc-4dd5-8924-0f5d01927b12
Bibby, Thomas S.
e04ea079-dd90-4ead-9840-00882de27ebd
Berepiki, Adokiye
1be9b7f8-011f-454c-bc9e-244b2c07799a
Hitchcock, Andrew
aeca86f3-e8cf-47d1-9338-e82630fb868e
Moore, Christopher Mark
7ec80b7b-bedc-4dd5-8924-0f5d01927b12
Bibby, Thomas S.
e04ea079-dd90-4ead-9840-00882de27ebd

Berepiki, Adokiye, Hitchcock, Andrew, Moore, Christopher Mark and Bibby, Thomas S. (2016) Tapping the unused potential of photosynthesis with a heterologous electron sink. ACS Synthetic Biology, 5 (12), 1369–1375. (doi:10.1021/acssynbio.6b00100).

Record type: Article

Abstract

Increasing the efficiency of the conversion of light energy to products by photosynthesis represents a grand challenge in biotechnology. Photosynthesis is limited by the carbon-fixing enzyme Rubisco resulting in much of the absorbed energy being wasted as heat, fluorescence or lost as excess reductant via alternative electron dissipation pathways. To harness this wasted reductant, we engineered the model cyanobacterium Synechococcus PCC 7002 to express the mammalian cytochrome P450 CYP1A1 to serve as an artificial electron sink for excess electrons derived from light-catalysed water-splitting. This improved photosynthetic efficiency by increasing the maximum rate of photosynthetic electron flow by 31.3%. A simple fluorescent assay for CYP1A1 activity demonstrated that the P450 was functional in the absence of its native reductase, that activity was light-dependent and scaled with irradiance. We show for the first time in live cells that photosynthetic reductant can be redirected to power a heterologous cytochrome P450. Furthermore, PCC 7002 expressing CYP1A1 degraded the herbicide atrazine, which is a widespread environmental pollutant.

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Berepiki%20et%20al%202016 - Accepted Manuscript
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Accepted/In Press date: 20 June 2016
e-pub ahead of print date: 20 July 2016
Organisations: Ocean and Earth Science

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Local EPrints ID: 398297
URI: http://eprints.soton.ac.uk/id/eprint/398297
PURE UUID: b5840608-2698-4b2b-8edb-e96f2677d8d4

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Date deposited: 22 Jul 2016 09:07
Last modified: 17 Dec 2019 06:17

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Contributors

Author: Adokiye Berepiki
Author: Andrew Hitchcock
Author: Thomas S. Bibby

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