Amperometric IFN-gamma immunosensors with commercially fabricated PCB sensing electrodes
Amperometric IFN-gamma immunosensors with commercially fabricated PCB sensing electrodes
Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve. The results demonstrate the potential for the integration of these components into an automated, disposable, electronic ELISA Lab-on-PCB diagnostic platform.
1-17
Moschou, Despina
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Greathead, Louise
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Pantelidis, Panagiotis
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Kelleher, Peter
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Morgan, Hywel
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Prodromakis, Themis
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Moschou, Despina
b5fef8ae-e4cb-4de5-8ade-1b5c807c74d3
Greathead, Louise
e7a0287f-ced3-4f69-a3ca-cc663e60c5cf
Pantelidis, Panagiotis
35ea018c-f5e2-498c-8741-84a66b5e2828
Kelleher, Peter
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Prodromakis, Themis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Moschou, Despina, Greathead, Louise, Pantelidis, Panagiotis, Kelleher, Peter, Morgan, Hywel and Prodromakis, Themis
(2016)
Amperometric IFN-gamma immunosensors with commercially fabricated PCB sensing electrodes.
Biosensors & Bioelectronics, .
(doi:10.1016/j.bios.2016.07.075).
Abstract
Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve. The results demonstrate the potential for the integration of these components into an automated, disposable, electronic ELISA Lab-on-PCB diagnostic platform.
Text
BiosensorsBioelectronics_Moschou et al_accepted.docx
- Accepted Manuscript
More information
Accepted/In Press date: 22 July 2016
e-pub ahead of print date: 25 July 2016
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 398447
URI: http://eprints.soton.ac.uk/id/eprint/398447
ISSN: 0956-5663
PURE UUID: bbdfffbb-a4d0-4db9-874f-a0d0374bfe74
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Date deposited: 26 Jul 2016 09:04
Last modified: 15 Mar 2024 05:45
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Contributors
Author:
Despina Moschou
Author:
Louise Greathead
Author:
Panagiotis Pantelidis
Author:
Peter Kelleher
Author:
Hywel Morgan
Author:
Themis Prodromakis
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