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High-velocity microsprays enhance antimicrobial activity in S. mutans biofilms

High-velocity microsprays enhance antimicrobial activity in S. mutans biofilms
High-velocity microsprays enhance antimicrobial activity in S. mutans biofilms
Streptococcus mutans in dental plaque biofilms play a role in caries development. The biofilm’s complex structure enhances the resistance to antimicrobial agents by limiting the transport of active agents inside the biofilm. We assessed the ability of high-velocity water microsprays to enhance delivery of antimicrobials into 3-days old S. mutans biofilms. Biofilms were exposed to a 90° or 30° impact, firstly using a 1-?m tracer beads solution (109 beads/mL) and secondly, a 0.2% Chlorhexidine (CHX) or 0.085% Cetylpyridinium chloride (CPC) solution. For comparison, a 30-sec diffusive transport and simulated mouthwash were also performed. Confocal microscopy was used to determine number and relative bead penetration depth (RD) into the biofilm. Assessment of antimicrobial penetration was determined by calculating the killing depth (KD) detected by live/dead viability staining. We firstly demonstrated that the microspray was able to deliver significantly more microbeads deeper in the biofilm compared to diffusion and mouthwashing exposures. Next our experiments revealed that the microspray yielded better antimicrobial penetration evidenced by deeper killing inside the biofilm and a wider killing zone around the zone of clearance than a diffusion transport with the same antimicrobials. Interestingly the 30° impact in the distal position delivered approximately 16 times more microbeads and yielded approximately 20% more bacteria killing (for both CHX and CPC) than the 90o impact. These data suggest that high-velocity water microsprays can be used as an effective mechanism to deliver micro-particles and antimicrobials inside S. mutans biofilms. High shear stresses generated at the biofilm/burst interface might have enhanced beads and antimicrobials delivery inside the remaining biofilm by combining forced advection into the biofilm matrix and physical restructuring of the biofilm itself. Further, the impact angle has potential to be optimized both for biofilm removal and active agents’ delivery inside biofilm in those protected areas where some biofilm might remain
plaque/plaque biofilms, dental hygiene, antimicrobials/antimicrobial resistance, caries detection/diagnosis/prevention, oral hygiene, drug delivery
0022-0345
1494-1500
Fabbri, S.
c93b6166-2117-48a9-9a88-b23a62c7b5da
Johnston, D.
b41163c9-b9d2-425c-af99-2a357204014e
Rmaile, A.
d6bb51ea-0892-465c-a2fe-5e530bc6330a
Gottenbos, B.
0b14ede8-eb0f-461c-a520-d36f290a4a08
De Jager, M.
97b2b3cb-02d5-4c02-9f12-49627b85b57c
Aspiras, M.
198433cd-5075-49d0-ac43-c305c35dc4f3
Starke, E.M.
52224939-da66-4070-afa6-01def56c4427
Ward, M.T.
21684a17-3051-43f9-bd3f-7adb147f22ab
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Fabbri, S.
c93b6166-2117-48a9-9a88-b23a62c7b5da
Johnston, D.
b41163c9-b9d2-425c-af99-2a357204014e
Rmaile, A.
d6bb51ea-0892-465c-a2fe-5e530bc6330a
Gottenbos, B.
0b14ede8-eb0f-461c-a520-d36f290a4a08
De Jager, M.
97b2b3cb-02d5-4c02-9f12-49627b85b57c
Aspiras, M.
198433cd-5075-49d0-ac43-c305c35dc4f3
Starke, E.M.
52224939-da66-4070-afa6-01def56c4427
Ward, M.T.
21684a17-3051-43f9-bd3f-7adb147f22ab
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f

Fabbri, S., Johnston, D., Rmaile, A., Gottenbos, B., De Jager, M., Aspiras, M., Starke, E.M., Ward, M.T. and Stoodley, P. (2016) High-velocity microsprays enhance antimicrobial activity in S. mutans biofilms. Journal of Dental Research, 95 (13), 1494-1500. (doi:10.1177/0022034516662813). (PMID:27554642)

Record type: Article

Abstract

Streptococcus mutans in dental plaque biofilms play a role in caries development. The biofilm’s complex structure enhances the resistance to antimicrobial agents by limiting the transport of active agents inside the biofilm. We assessed the ability of high-velocity water microsprays to enhance delivery of antimicrobials into 3-days old S. mutans biofilms. Biofilms were exposed to a 90° or 30° impact, firstly using a 1-?m tracer beads solution (109 beads/mL) and secondly, a 0.2% Chlorhexidine (CHX) or 0.085% Cetylpyridinium chloride (CPC) solution. For comparison, a 30-sec diffusive transport and simulated mouthwash were also performed. Confocal microscopy was used to determine number and relative bead penetration depth (RD) into the biofilm. Assessment of antimicrobial penetration was determined by calculating the killing depth (KD) detected by live/dead viability staining. We firstly demonstrated that the microspray was able to deliver significantly more microbeads deeper in the biofilm compared to diffusion and mouthwashing exposures. Next our experiments revealed that the microspray yielded better antimicrobial penetration evidenced by deeper killing inside the biofilm and a wider killing zone around the zone of clearance than a diffusion transport with the same antimicrobials. Interestingly the 30° impact in the distal position delivered approximately 16 times more microbeads and yielded approximately 20% more bacteria killing (for both CHX and CPC) than the 90o impact. These data suggest that high-velocity water microsprays can be used as an effective mechanism to deliver micro-particles and antimicrobials inside S. mutans biofilms. High shear stresses generated at the biofilm/burst interface might have enhanced beads and antimicrobials delivery inside the remaining biofilm by combining forced advection into the biofilm matrix and physical restructuring of the biofilm itself. Further, the impact angle has potential to be optimized both for biofilm removal and active agents’ delivery inside biofilm in those protected areas where some biofilm might remain

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More information

Accepted/In Press date: 14 July 2016
e-pub ahead of print date: 23 August 2016
Published date: December 2016
Keywords: plaque/plaque biofilms, dental hygiene, antimicrobials/antimicrobial resistance, caries detection/diagnosis/prevention, oral hygiene, drug delivery
Organisations: Faculty of Medicine, Faculty of Health Sciences, Bioengineering Group, nCATS Group

Identifiers

Local EPrints ID: 398601
URI: http://eprints.soton.ac.uk/id/eprint/398601
ISSN: 0022-0345
PURE UUID: aea858a3-589e-4b09-83f5-7a7952bb7cd0
ORCID for D. Johnston: ORCID iD orcid.org/0000-0001-6703-6014
ORCID for P. Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 29 Jul 2016 12:13
Last modified: 18 Feb 2021 17:13

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