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Development of a multilocus sequence typing scheme for molecular typing of Mycoplasma pneumoniae

Development of a multilocus sequence typing scheme for molecular typing of Mycoplasma pneumoniae
Development of a multilocus sequence typing scheme for molecular typing of Mycoplasma pneumoniae
Mycoplasma pneumoniae is a major human respiratory pathogen causing both upper and lower respiratory disease in humans of all ages, and it can also result in other serious extrapulmonary sequelae. A multilocus sequence typing (MLST) scheme for M. pneumoniae was developed based on the sequences of eight housekeeping genes (ppa, pgm, gyrB, gmk, glyA, atpA, arcC, and adk) and applied to 55 M. pneumoniae clinical isolates and the two type strains M129 and FH. A total of 12 sequence types (STs) resulted for 57 M. pneumoniae isolates tested, with a discriminatory index of 0.21 STs per isolate. The MLST loci used in this scheme were shown to be stable in 10 strains following 10 sequential subculture passages. Phylogenetic analysis of concatenated sequences of the eight loci indicated two distinct genetic clusters that were directly linked to multilocus variable-number tandem repeat analysis (MLVA) type. Genetic MLST clustering was confirmed by genomic sequence analysis, indicating that the MLST scheme developed in this study is representative of the genome. Furthermore, this MLST scheme was shown to be more discriminatory than both MLVA and P1 typing for the M. pneumoniae isolates examined, providing a method for further and more detailed analysis of observed epidemic peaks of M. pneumoniae infection. This scheme is supported by a public Web-based database (http://pubmlst.org/mpneumoniae).
0095-1137
3195-3203
Brown, Rebecca
a4912e7b-0056-4d4d-843d-3baf69f97f58
Holden, Matthew
0efa6225-728a-4738-977a-7b0a0c2c699b
Spiller, O. Brad
a802a26a-804c-4839-a25e-830e3856e852
Chalker, Victoria
7e7a099b-3b9a-4f62-b8d3-b8e469cb1541
Brown, Rebecca
a4912e7b-0056-4d4d-843d-3baf69f97f58
Holden, Matthew
0efa6225-728a-4738-977a-7b0a0c2c699b
Spiller, O. Brad
a802a26a-804c-4839-a25e-830e3856e852
Chalker, Victoria
7e7a099b-3b9a-4f62-b8d3-b8e469cb1541

Brown, Rebecca, Holden, Matthew, Spiller, O. Brad and Chalker, Victoria (2015) Development of a multilocus sequence typing scheme for molecular typing of Mycoplasma pneumoniae. Journal of Clinical Microbiology, 53 (10), 3195-3203. (doi:10.1128/JCM.01301-15).

Record type: Article

Abstract

Mycoplasma pneumoniae is a major human respiratory pathogen causing both upper and lower respiratory disease in humans of all ages, and it can also result in other serious extrapulmonary sequelae. A multilocus sequence typing (MLST) scheme for M. pneumoniae was developed based on the sequences of eight housekeeping genes (ppa, pgm, gyrB, gmk, glyA, atpA, arcC, and adk) and applied to 55 M. pneumoniae clinical isolates and the two type strains M129 and FH. A total of 12 sequence types (STs) resulted for 57 M. pneumoniae isolates tested, with a discriminatory index of 0.21 STs per isolate. The MLST loci used in this scheme were shown to be stable in 10 strains following 10 sequential subculture passages. Phylogenetic analysis of concatenated sequences of the eight loci indicated two distinct genetic clusters that were directly linked to multilocus variable-number tandem repeat analysis (MLVA) type. Genetic MLST clustering was confirmed by genomic sequence analysis, indicating that the MLST scheme developed in this study is representative of the genome. Furthermore, this MLST scheme was shown to be more discriminatory than both MLVA and P1 typing for the M. pneumoniae isolates examined, providing a method for further and more detailed analysis of observed epidemic peaks of M. pneumoniae infection. This scheme is supported by a public Web-based database (http://pubmlst.org/mpneumoniae).

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Accepted/In Press date: 14 July 2015
e-pub ahead of print date: 22 July 2015
Published date: October 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 399079
URI: https://eprints.soton.ac.uk/id/eprint/399079
ISSN: 0095-1137
PURE UUID: 7fdf02fc-3700-4c68-86a8-6a823625a854
ORCID for Rebecca Brown: ORCID iD orcid.org/0000-0001-5825-6859

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Date deposited: 05 Aug 2016 08:17
Last modified: 20 Jul 2019 00:33

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Contributors

Author: Rebecca Brown ORCID iD
Author: Matthew Holden
Author: O. Brad Spiller
Author: Victoria Chalker

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