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Nanoparticles modulate surfactant protein A and D mediated protection against influenza A infection in vitro

Nanoparticles modulate surfactant protein A and D mediated protection against influenza A infection in vitro
Nanoparticles modulate surfactant protein A and D mediated protection against influenza A infection in vitro
Numerous epidemiological and toxicological studies have indicated that respiratory infections are exacerbated following enhanced exposure to airborne particulates. Surfactant protein A (SP-A) and SP-D form an important part of the innate immune response in the lung and can interact with nanoparticles to modulate the cellular uptake of these particles. We hypothesize that this interaction will also affect the ability of these proteins to combat infections. TT1, A549 and differentiated THP-1 cells, representing the predominant cell types found in the alveolus namely alveolar type I (ATI) epithelial cells, ATII cells and macrophages, were used to examine the effect of two model nanoparticles, 100 nm amine modified (A-PS) and unmodified polystyrene (U-PS), on the ability of SP-A and SP-D to neutralize influenza A infections in vitro. Pre-incubation of low concentrations of U-PS with SP-A resulted in a reduction of SP-A anti-influenza activity in A549 cells, whereas at higher concentrations there was an increase in SP-A antiviral activity. This differential pattern of U-PS concentration on surfactant protein mediated protection against IAV was also shown with SP-D in TT1 cells. On the other hand, low concentrations of A-PS particles resulted in a reduction of SP-A activity in TT1 cells and a reduction in SP-D activity in A549 cells. These results indicate that nanoparticles can modulate the ability of SP-A and SP-D to combat viral challenges. Furthermore, the nanoparticle concentration, surface chemistry and cell type under investigation are important factors in determining the extent of these modulations.
0962-8436
1-8
Mckenzie, Zofi
b4f47115-f00c-4f6c-8e54-030da0da0736
Kendall, Michaela
ec95f244-5349-4521-a60f-4a60648f6de3
Mackay, Rose-Marie
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Tetley, Teresa D.
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Morgan, Cliff
74b0ee12-7045-44d3-bfe7-1118bbdfe463
Griffiths, Mark
ff46d26f-2a28-4a6a-b189-d838abfcf8f7
Clark, Howard W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Mckenzie, Zofi
b4f47115-f00c-4f6c-8e54-030da0da0736
Kendall, Michaela
ec95f244-5349-4521-a60f-4a60648f6de3
Mackay, Rose-Marie
19cf1b92-c65d-4baa-a165-ab630bf77ec3
Tetley, Teresa D.
85489d1b-9012-451c-97f2-82368c9dd081
Morgan, Cliff
74b0ee12-7045-44d3-bfe7-1118bbdfe463
Griffiths, Mark
ff46d26f-2a28-4a6a-b189-d838abfcf8f7
Clark, Howard W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359

Mckenzie, Zofi, Kendall, Michaela, Mackay, Rose-Marie, Tetley, Teresa D., Morgan, Cliff, Griffiths, Mark, Clark, Howard W. and Madsen, Jens (2015) Nanoparticles modulate surfactant protein A and D mediated protection against influenza A infection in vitro. Philosophical Transactions of The Royal Society B Biological Sciences, 370 (1661), 1-8. (doi:10.1098/rstb.2014.0049). (PMID:19017984)

Record type: Article

Abstract

Numerous epidemiological and toxicological studies have indicated that respiratory infections are exacerbated following enhanced exposure to airborne particulates. Surfactant protein A (SP-A) and SP-D form an important part of the innate immune response in the lung and can interact with nanoparticles to modulate the cellular uptake of these particles. We hypothesize that this interaction will also affect the ability of these proteins to combat infections. TT1, A549 and differentiated THP-1 cells, representing the predominant cell types found in the alveolus namely alveolar type I (ATI) epithelial cells, ATII cells and macrophages, were used to examine the effect of two model nanoparticles, 100 nm amine modified (A-PS) and unmodified polystyrene (U-PS), on the ability of SP-A and SP-D to neutralize influenza A infections in vitro. Pre-incubation of low concentrations of U-PS with SP-A resulted in a reduction of SP-A anti-influenza activity in A549 cells, whereas at higher concentrations there was an increase in SP-A antiviral activity. This differential pattern of U-PS concentration on surfactant protein mediated protection against IAV was also shown with SP-D in TT1 cells. On the other hand, low concentrations of A-PS particles resulted in a reduction of SP-A activity in TT1 cells and a reduction in SP-D activity in A549 cells. These results indicate that nanoparticles can modulate the ability of SP-A and SP-D to combat viral challenges. Furthermore, the nanoparticle concentration, surface chemistry and cell type under investigation are important factors in determining the extent of these modulations.

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More information

e-pub ahead of print date: 22 December 2014
Published date: 5 February 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 399245
URI: https://eprints.soton.ac.uk/id/eprint/399245
ISSN: 0962-8436
PURE UUID: 24691b05-c44c-46ca-9537-2a982de54090
ORCID for Rose-Marie Mackay: ORCID iD orcid.org/0000-0002-9493-9654
ORCID for Jens Madsen: ORCID iD orcid.org/0000-0003-1664-7645

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Date deposited: 10 Aug 2016 11:04
Last modified: 06 Jun 2018 12:38

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Contributors

Author: Zofi Mckenzie
Author: Michaela Kendall
Author: Rose-Marie Mackay ORCID iD
Author: Teresa D. Tetley
Author: Cliff Morgan
Author: Mark Griffiths
Author: Howard W. Clark
Author: Jens Madsen ORCID iD

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