The University of Southampton
University of Southampton Institutional Repository

Nanoparticles in the lung and their protein corona: the few proteins that count

Nanoparticles in the lung and their protein corona: the few proteins that count
Nanoparticles in the lung and their protein corona: the few proteins that count
The formation of protein coronas on nanoparticles (NP) has been investigated almost exclusively in serum, despite the prevailing route of exposure being inhalation of airborne particles. In addition, an increasing number of nanomedicines, that exploit the airways as the site of delivery, are undergoing medical trials. An understanding of the effects of NPs on the airways is therefore required. To further this field, we have described the corona formed on polystyrene particles with different surface modifications and on titanium dioxide particles when incubated in human bronchoalveolar lavage fluid (BALF) from patients with protein alveolar proteinosis (PAP). We show, using high-resolution quantitative mass spectrometry (MS(E)), that a large number of proteins bind with low copy numbers but that a few 'core' proteins bind to all particles tested with high fidelity, averaging the surface properties of the different particles independent of the surface properties of the specific particle. The averaging effect at the particle surface means that differing cellular effects may not be due to the protein corona but due to the surface properties of the nanoparticle once inside the cell. Finally, the adherence of surfactant associated proteins (SP-A, B and D) suggests that there may be interactions with lipids and pulmonary surfactant (PSf), which could have potential in vivo health effects for people with chronic airway diseases such as asthma and chronic pulmonary obstructive disease (COPD), who have increased susceptibility toward other respiratory diseases.
nanoparticles, surfactant, lung, corona, surface chemistry
1743-5390
1-24
Whitwell, Harry
94b6f20c-7924-4b33-8973-010616da9bcf
Mackay, Rose-Marie
19cf1b92-c65d-4baa-a165-ab630bf77ec3
Elgy, Christine
9dc5e317-7809-46ba-9e70-75bcc9700ff5
Morgan, Cliff
74b0ee12-7045-44d3-bfe7-1118bbdfe463
Griffiths, Mark
ff46d26f-2a28-4a6a-b189-d838abfcf8f7
Clark, Howard
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Skipp, Paul
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Whitwell, Harry
94b6f20c-7924-4b33-8973-010616da9bcf
Mackay, Rose-Marie
19cf1b92-c65d-4baa-a165-ab630bf77ec3
Elgy, Christine
9dc5e317-7809-46ba-9e70-75bcc9700ff5
Morgan, Cliff
74b0ee12-7045-44d3-bfe7-1118bbdfe463
Griffiths, Mark
ff46d26f-2a28-4a6a-b189-d838abfcf8f7
Clark, Howard
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Skipp, Paul
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359

Whitwell, Harry, Mackay, Rose-Marie, Elgy, Christine, Morgan, Cliff, Griffiths, Mark, Clark, Howard, Skipp, Paul and Madsen, Jens (2016) Nanoparticles in the lung and their protein corona: the few proteins that count. Nanotoxicology, 1-24. (doi:10.1080/17435390.2016.1218080). (PMID:27465202)

Record type: Article

Abstract

The formation of protein coronas on nanoparticles (NP) has been investigated almost exclusively in serum, despite the prevailing route of exposure being inhalation of airborne particles. In addition, an increasing number of nanomedicines, that exploit the airways as the site of delivery, are undergoing medical trials. An understanding of the effects of NPs on the airways is therefore required. To further this field, we have described the corona formed on polystyrene particles with different surface modifications and on titanium dioxide particles when incubated in human bronchoalveolar lavage fluid (BALF) from patients with protein alveolar proteinosis (PAP). We show, using high-resolution quantitative mass spectrometry (MS(E)), that a large number of proteins bind with low copy numbers but that a few 'core' proteins bind to all particles tested with high fidelity, averaging the surface properties of the different particles independent of the surface properties of the specific particle. The averaging effect at the particle surface means that differing cellular effects may not be due to the protein corona but due to the surface properties of the nanoparticle once inside the cell. Finally, the adherence of surfactant associated proteins (SP-A, B and D) suggests that there may be interactions with lipids and pulmonary surfactant (PSf), which could have potential in vivo health effects for people with chronic airway diseases such as asthma and chronic pulmonary obstructive disease (COPD), who have increased susceptibility toward other respiratory diseases.

Text
WHITWELL - Nanotoxicology -Nanoparticles in lung and their protein corona.pdf - Accepted Manuscript
Download (1MB)
Text
Manuscript WHITWELL Nanoparticles in the Lung_Nanotox shorter with figures July 2016.pdf - Author's Original
Download (2MB)

More information

Accepted/In Press date: 25 July 2016
e-pub ahead of print date: 28 July 2016
Keywords: nanoparticles, surfactant, lung, corona, surface chemistry
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 399247
URI: https://eprints.soton.ac.uk/id/eprint/399247
ISSN: 1743-5390
PURE UUID: 68fca0d8-4f7e-4b02-946f-e6e5d56d77ea
ORCID for Rose-Marie Mackay: ORCID iD orcid.org/0000-0002-9493-9654
ORCID for Paul Skipp: ORCID iD orcid.org/0000-0002-2995-2959
ORCID for Jens Madsen: ORCID iD orcid.org/0000-0003-1664-7645

Catalogue record

Date deposited: 10 Aug 2016 15:21
Last modified: 10 Dec 2019 06:27

Export record

Altmetrics

Contributors

Author: Harry Whitwell
Author: Rose-Marie Mackay ORCID iD
Author: Christine Elgy
Author: Cliff Morgan
Author: Mark Griffiths
Author: Howard Clark
Author: Paul Skipp ORCID iD
Author: Jens Madsen ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×