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Ionizing radiation-induced DNA damage response identified in marine mussels, Mytilus sp

Ionizing radiation-induced DNA damage response identified in marine mussels, Mytilus sp
Ionizing radiation-induced DNA damage response identified in marine mussels, Mytilus sp
There is growing concern over the potential detrimental impact of ionizing radiation on natural biota. The mechanistic cause-and-effect impact of ionizing radiation has yet to be characterized in any aquatic species. Adopting an integrated approach, including radiochemical analysis of environmental samples, we evaluate molecular responses to ionizing radiation in the marine mussel, Mytilus edulis. These responses included analyses of RAD51 mRNA expression, a gene involved in the repair of DNA double strand breaks, and induction of DNA strand breaks using the comet assay, in samples collected from a site impacted by low level ionizing radiation discharges. Based on activities of the radionuclides measured in sediment and mussel tissue at the discharge site, external and internal dose rates were low, at ca. 0.61 ?Gyh?1 and significantly lower than the generic (all species) “no effect” dose rate of 10 uGyh?1, yet DNA strand breakage and RAD51 mRNA expression were both altered.
RAD51, DNA damage & repair, Ionizing radiation, Mussels
0269-7491
107-112
AlAmri, Ohoud D.
19f0a385-ae09-41f7-b31d-b8f1db2347fd
Cundy, Andrew B.
994fdc96-2dce-40f4-b74b-dc638286eb08
Di, Yanan
02105810-1e3e-4f65-884c-e6429a6d0ffb
Jha, Awadhesh N.
d1809263-cf3d-4cd2-936c-90a8307e5609
Rotchell, Jeanette M.
1d8d7d58-0774-4c30-adba-6118938ebb54
AlAmri, Ohoud D.
19f0a385-ae09-41f7-b31d-b8f1db2347fd
Cundy, Andrew B.
994fdc96-2dce-40f4-b74b-dc638286eb08
Di, Yanan
02105810-1e3e-4f65-884c-e6429a6d0ffb
Jha, Awadhesh N.
d1809263-cf3d-4cd2-936c-90a8307e5609
Rotchell, Jeanette M.
1d8d7d58-0774-4c30-adba-6118938ebb54

AlAmri, Ohoud D., Cundy, Andrew B., Di, Yanan, Jha, Awadhesh N. and Rotchell, Jeanette M. (2012) Ionizing radiation-induced DNA damage response identified in marine mussels, Mytilus sp. Environmental Pollution, 168, 107-112. (doi:10.1016/j.envpol.2012.04.015).

Record type: Article

Abstract

There is growing concern over the potential detrimental impact of ionizing radiation on natural biota. The mechanistic cause-and-effect impact of ionizing radiation has yet to be characterized in any aquatic species. Adopting an integrated approach, including radiochemical analysis of environmental samples, we evaluate molecular responses to ionizing radiation in the marine mussel, Mytilus edulis. These responses included analyses of RAD51 mRNA expression, a gene involved in the repair of DNA double strand breaks, and induction of DNA strand breaks using the comet assay, in samples collected from a site impacted by low level ionizing radiation discharges. Based on activities of the radionuclides measured in sediment and mussel tissue at the discharge site, external and internal dose rates were low, at ca. 0.61 ?Gyh?1 and significantly lower than the generic (all species) “no effect” dose rate of 10 uGyh?1, yet DNA strand breakage and RAD51 mRNA expression were both altered.

Full text not available from this repository.

More information

Published date: September 2012
Keywords: RAD51, DNA damage & repair, Ionizing radiation, Mussels
Organisations: Geochemistry

Identifiers

Local EPrints ID: 399295
URI: https://eprints.soton.ac.uk/id/eprint/399295
ISSN: 0269-7491
PURE UUID: 3533f71e-c2d6-47ad-afbe-1bf5055a1938
ORCID for Andrew B. Cundy: ORCID iD orcid.org/0000-0003-4368-2569

Catalogue record

Date deposited: 10 Aug 2016 15:57
Last modified: 29 Oct 2019 01:35

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