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Primary air–liquid interface culture of nasal epithelium for nasal drug delivery

Primary air–liquid interface culture of nasal epithelium for nasal drug delivery
Primary air–liquid interface culture of nasal epithelium for nasal drug delivery
Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air–liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.
1543-8384
2242-2252
Ong, Hui Xin
f2241b82-9866-4737-afdf-3e99faa43c60
Jackson, Claire L.
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Cole, Janice L.
fb9d20aa-93b9-42b3-9b9e-bab2f565ea60
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Traini, Daniela
ed203afd-8e53-427c-a473-3c1cd26c23ea
Young, Paul M.
17827d88-bd0f-470b-af66-88f73b378917
Lucas, Jane
5cb3546c-87b2-4e59-af48-402076e25313
Conway, Joy
bbe9a2e4-fb85-4d4a-a38c-0c1832c32d06
Ong, Hui Xin
f2241b82-9866-4737-afdf-3e99faa43c60
Jackson, Claire L.
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Cole, Janice L.
fb9d20aa-93b9-42b3-9b9e-bab2f565ea60
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Traini, Daniela
ed203afd-8e53-427c-a473-3c1cd26c23ea
Young, Paul M.
17827d88-bd0f-470b-af66-88f73b378917
Lucas, Jane
5cb3546c-87b2-4e59-af48-402076e25313
Conway, Joy
bbe9a2e4-fb85-4d4a-a38c-0c1832c32d06

Ong, Hui Xin, Jackson, Claire L., Cole, Janice L., Lackie, Peter M., Traini, Daniela, Young, Paul M., Lucas, Jane and Conway, Joy (2016) Primary air–liquid interface culture of nasal epithelium for nasal drug delivery. Molecular Pharmaceutics, 13 (7), 2242-2252. (doi:10.1021/acs.molpharmaceut.5b00852).

Record type: Article

Abstract

Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air–liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.

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More information

Accepted/In Press date: 25 May 2016
e-pub ahead of print date: 25 May 2016
Published date: 5 July 2016
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 399414
URI: http://eprints.soton.ac.uk/id/eprint/399414
ISSN: 1543-8384
PURE UUID: 11e49de9-c221-4bb1-b945-fd1458839359
ORCID for Peter M. Lackie: ORCID iD orcid.org/0000-0001-7138-3764
ORCID for Joy Conway: ORCID iD orcid.org/0000-0001-6464-1526

Catalogue record

Date deposited: 16 Aug 2016 08:58
Last modified: 26 Nov 2021 02:36

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Contributors

Author: Hui Xin Ong
Author: Claire L. Jackson
Author: Janice L. Cole
Author: Peter M. Lackie ORCID iD
Author: Daniela Traini
Author: Paul M. Young
Author: Jane Lucas
Author: Joy Conway ORCID iD

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