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Stabilisation of self-assembled DNA crystals by triplex-directed photo-cross-linking

Stabilisation of self-assembled DNA crystals by triplex-directed photo-cross-linking
Stabilisation of self-assembled DNA crystals by triplex-directed photo-cross-linking
The tensegrity triangle is a robust DNA motif that can self-assemble to generate macroscopic three-dimensional crystals. However, the stability of these crystals is dependent on the high ionic conditions used for crystal growth. Here we demonstrate that a triplex-forming oligonucleotide can be used to direct the specific intercalation, and subsequent photo-cross-linking, of 4,5?,8-trimethylpsoralen to single or multiple loci within or between the tiles of the crystal. Cross-linking between the tiles of the crystal improves their thermal stability. Such an approach is likely to facilitate the removal of crystals from their mother liquor and may prove useful for applications that require greater crystal stability.
DNA, self-assembly, recognition, crystals, stability
1359-7345
8014-8017
Abdallah, Hatem O.
e02a7492-4029-4524-a154-092dc1eac8a0
Ohayon, Yoel P.
976fbcb0-f244-443d-9dc9-ae5df7c563c1
Chandrasekaran, Arun Richard
96e2438e-04e3-442e-9a62-8f6199535faf
Sha, Ruojie
f735bfa2-e9cd-4c77-8988-af46e576c2c2
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Mao, Chengde
cd6009d7-d766-4aa0-a4e7-f04f2bdc9f3c
Seeman, Nadrian C.
ace19c8f-2353-465e-acfa-366a6ca6179b
Abdallah, Hatem O.
e02a7492-4029-4524-a154-092dc1eac8a0
Ohayon, Yoel P.
976fbcb0-f244-443d-9dc9-ae5df7c563c1
Chandrasekaran, Arun Richard
96e2438e-04e3-442e-9a62-8f6199535faf
Sha, Ruojie
f735bfa2-e9cd-4c77-8988-af46e576c2c2
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Mao, Chengde
cd6009d7-d766-4aa0-a4e7-f04f2bdc9f3c
Seeman, Nadrian C.
ace19c8f-2353-465e-acfa-366a6ca6179b

Abdallah, Hatem O., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Sha, Ruojie, Fox, Keith R., Brown, Tom, Rusling, David A., Mao, Chengde and Seeman, Nadrian C. (2016) Stabilisation of self-assembled DNA crystals by triplex-directed photo-cross-linking. Chemical Communications, 52 (51), 8014-8017. (doi:10.1039/c6cc03695c).

Record type: Article

Abstract

The tensegrity triangle is a robust DNA motif that can self-assemble to generate macroscopic three-dimensional crystals. However, the stability of these crystals is dependent on the high ionic conditions used for crystal growth. Here we demonstrate that a triplex-forming oligonucleotide can be used to direct the specific intercalation, and subsequent photo-cross-linking, of 4,5?,8-trimethylpsoralen to single or multiple loci within or between the tiles of the crystal. Cross-linking between the tiles of the crystal improves their thermal stability. Such an approach is likely to facilitate the removal of crystals from their mother liquor and may prove useful for applications that require greater crystal stability.

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More information

Accepted/In Press date: 18 May 2016
e-pub ahead of print date: 6 June 2016
Published date: 6 June 2016
Keywords: DNA, self-assembly, recognition, crystals, stability
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 399714
URI: https://eprints.soton.ac.uk/id/eprint/399714
ISSN: 1359-7345
PURE UUID: adb6868c-7452-4662-8e61-87cc7db9c5b7
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315
ORCID for David A. Rusling: ORCID iD orcid.org/0000-0002-7442-686X

Catalogue record

Date deposited: 25 Aug 2016 10:05
Last modified: 06 Jun 2018 13:16

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