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The chorioallantoic membrane (CAM) assay for the study of human bone regeneration: a refinement animal model for tissue engineering.

The chorioallantoic membrane (CAM) assay for the study of human bone regeneration: a refinement animal model for tissue engineering.
The chorioallantoic membrane (CAM) assay for the study of human bone regeneration: a refinement animal model for tissue engineering.
Biomaterial development for tissue engineering applications is rapidly increasing but necessitates efficacy and safety testing prior to clinical application. Current in vitro and in vivo models hold a number of limitations, including expense, lack of correlation between animal models and human outcomes and the need to perform invasive procedures on animals; hence requiring new predictive screening methods. In the present study we tested the hypothesis that the chick embryo chorioallantoic membrane (CAM) can be used as a bioreactor to culture and study the regeneration of human living bone. We extracted bone cylinders from human femoral heads, simulated an injury using a drill-hole defect, and implanted the bone on CAM or in vitro control-culture. Micro-computed tomography (?CT) was used to quantify the magnitude and location of bone volume changes followed by histological analyses to assess bone repair. CAM blood vessels were observed to infiltrate the human bone cylinder and maintain human cell viability. Histological evaluation revealed extensive extracellular matrix deposition in proximity to endochondral condensations (Sox9+) on the CAM-implanted bone cylinders, correlating with a significant increase in bone volume by ?CT analysis (p?<?0.01). This human-avian system offers a simple refinement model for animal research and a step towards a humanized in vivo model for tissue engineering.
Moreno-Jiménez, Ines
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Hulsart Billstrom, Gry
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Lanham, Stuart A.
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Janeczek, Agnieszka A.
81b58bec-079a-4484-b855-ec01ca49ec60
Kontouli, Nasia
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Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Evans, Nicholas D.
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Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Moreno-Jiménez, Ines
4dbe5fd4-8b2b-48c6-8c48-4833fd7d7d50
Hulsart Billstrom, Gry
41799212-48c4-4791-9a24-9090a77ba6cc
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Janeczek, Agnieszka A.
81b58bec-079a-4484-b855-ec01ca49ec60
Kontouli, Nasia
63602628-7753-421d-817e-071cd752b848
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Moreno-Jiménez, Ines, Hulsart Billstrom, Gry, Lanham, Stuart A., Janeczek, Agnieszka A., Kontouli, Nasia, Kanczler, Janos M., Evans, Nicholas D. and Oreffo, Richard O.C. (2016) The chorioallantoic membrane (CAM) assay for the study of human bone regeneration: a refinement animal model for tissue engineering. Scientific Reports, 6 (32168). (doi:10.1038/srep32168). (PMID:27577960)

Record type: Article

Abstract

Biomaterial development for tissue engineering applications is rapidly increasing but necessitates efficacy and safety testing prior to clinical application. Current in vitro and in vivo models hold a number of limitations, including expense, lack of correlation between animal models and human outcomes and the need to perform invasive procedures on animals; hence requiring new predictive screening methods. In the present study we tested the hypothesis that the chick embryo chorioallantoic membrane (CAM) can be used as a bioreactor to culture and study the regeneration of human living bone. We extracted bone cylinders from human femoral heads, simulated an injury using a drill-hole defect, and implanted the bone on CAM or in vitro control-culture. Micro-computed tomography (?CT) was used to quantify the magnitude and location of bone volume changes followed by histological analyses to assess bone repair. CAM blood vessels were observed to infiltrate the human bone cylinder and maintain human cell viability. Histological evaluation revealed extensive extracellular matrix deposition in proximity to endochondral condensations (Sox9+) on the CAM-implanted bone cylinders, correlating with a significant increase in bone volume by ?CT analysis (p?<?0.01). This human-avian system offers a simple refinement model for animal research and a step towards a humanized in vivo model for tissue engineering.

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Accepted/In Press date: 2 August 2016
e-pub ahead of print date: 31 August 2016
Organisations: Human Development & Health

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Local EPrints ID: 399874
URI: http://eprints.soton.ac.uk/id/eprint/399874
PURE UUID: 59446fb8-18d4-4ccd-92c2-81340fcfc7fe
ORCID for Stuart A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Janos M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Nicholas D. Evans: ORCID iD orcid.org/0000-0002-3255-4388
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 02 Sep 2016 09:54
Last modified: 12 Jul 2024 01:48

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Contributors

Author: Ines Moreno-Jiménez
Author: Gry Hulsart Billstrom
Author: Stuart A. Lanham ORCID iD
Author: Agnieszka A. Janeczek
Author: Nasia Kontouli
Author: Janos M. Kanczler ORCID iD

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