Open Access UHPSFC/MS - an additional analytical resource for an academic mass spectrometry facility

Abstract

RATIONALE: Many compounds submitted for analysis in Chemistry at the University of Southampton either do not retain, elute or ionise using open access RP-UHPLC/MS and required analysis via infusion. An ultra-high performance supercritical fluid chromatography mass spectrometry approach was implemented to afford high through-put analysis of these compounds with chromatographic separation.

METHODS: A UPC2-TQD MS system has been incorporated into the open access MS provision within Chemistry at the University of Southampton, using an ESCi source (electrospray and atmospheric pressure chemical ionization) and an atmospheric pressure photoionization (APPI) source. Access to instrumentation is enabled via a web-based interface (RemoteAnalyzer™).

RESULTS: Compounds such as fluorosugars, fullerenes, phosphoramidites, porphyrins, and rotaxanes exhibiting properties incompatible with RP-UHPLC/MS have been analysed using automated chromatography and mass spectrometry methods. The speedy return of data enables research in these areas to progress unhindered by sample type. The provision of an electronic web format enables easy incorporation of chromatograms and mass spectra into electronic files and reports.

CONCLUSIONS: The implementation of UHPSFC/MS increases access to a wide range of chemistries incompatible with reversed-phase chromatography and polar solvents, enabling more than 90% of submitted samples to be analysed using an open access approach. Further, chromatographic separation is provided where previously flow injection or infusion analysis were the only options.

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Identifiers

ORCID for Julie M. Herniman: orcid.org/0000-0003-4834-1093

ORCID for G. John Langley: orcid.org/0000-0002-8323-7235

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