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Open Access UHPSFC/MS - an additional analytical resource for an academic mass spectrometry facility

Open Access UHPSFC/MS - an additional analytical resource for an academic mass spectrometry facility
Open Access UHPSFC/MS - an additional analytical resource for an academic mass spectrometry facility
RATIONALE: Many compounds submitted for analysis in Chemistry at the University of Southampton either do not retain, elute or ionise using open access RP-UHPLC/MS and required analysis via infusion. An ultra-high performance supercritical fluid chromatography mass spectrometry approach was implemented to afford high through-put analysis of these compounds with chromatographic separation.

METHODS: A UPC2-TQD MS system has been incorporated into the open access MS provision within Chemistry at the University of Southampton, using an ESCi source (electrospray and atmospheric pressure chemical ionization) and an atmospheric pressure photoionization (APPI) source. Access to instrumentation is enabled via a web-based interface (RemoteAnalyzer™).

RESULTS: Compounds such as fluorosugars, fullerenes, phosphoramidites, porphyrins, and rotaxanes exhibiting properties incompatible with RP-UHPLC/MS have been analysed using automated chromatography and mass spectrometry methods. The speedy return of data enables research in these areas to progress unhindered by sample type. The provision of an electronic web format enables easy incorporation of chromatograms and mass spectra into electronic files and reports.

CONCLUSIONS: The implementation of UHPSFC/MS increases access to a wide range of chemistries incompatible with reversed-phase chromatography and polar solvents, enabling more than 90% of submitted samples to be analysed using an open access approach. Further, chromatographic separation is provided where previously flow injection or infusion analysis were the only options.
0951-4198
1811-1817
Herniman, Julie M.
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Herniman, Julie M.
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea

Herniman, Julie M. and Langley, G. John (2016) Open Access UHPSFC/MS - an additional analytical resource for an academic mass spectrometry facility. Rapid Communications in Mass Spectrometry, 30 (15), 1811-1817. (doi:10.1002/rcm.7660).

Record type: Article

Abstract

RATIONALE: Many compounds submitted for analysis in Chemistry at the University of Southampton either do not retain, elute or ionise using open access RP-UHPLC/MS and required analysis via infusion. An ultra-high performance supercritical fluid chromatography mass spectrometry approach was implemented to afford high through-put analysis of these compounds with chromatographic separation.

METHODS: A UPC2-TQD MS system has been incorporated into the open access MS provision within Chemistry at the University of Southampton, using an ESCi source (electrospray and atmospheric pressure chemical ionization) and an atmospheric pressure photoionization (APPI) source. Access to instrumentation is enabled via a web-based interface (RemoteAnalyzer™).

RESULTS: Compounds such as fluorosugars, fullerenes, phosphoramidites, porphyrins, and rotaxanes exhibiting properties incompatible with RP-UHPLC/MS have been analysed using automated chromatography and mass spectrometry methods. The speedy return of data enables research in these areas to progress unhindered by sample type. The provision of an electronic web format enables easy incorporation of chromatograms and mass spectra into electronic files and reports.

CONCLUSIONS: The implementation of UHPSFC/MS increases access to a wide range of chemistries incompatible with reversed-phase chromatography and polar solvents, enabling more than 90% of submitted samples to be analysed using an open access approach. Further, chromatographic separation is provided where previously flow injection or infusion analysis were the only options.

Text
Open Access UHPSFCMS Herniman and Langley changes accepted 31 May 2016.pdf - Accepted Manuscript
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More information

Accepted/In Press date: 7 June 2016
e-pub ahead of print date: 7 July 2016
Published date: 15 August 2016
Organisations: Chemistry, Faculty of Natural and Environmental Sciences

Identifiers

Local EPrints ID: 399889
URI: http://eprints.soton.ac.uk/id/eprint/399889
ISSN: 0951-4198
PURE UUID: 8d67b569-a6d2-4d49-99ed-80e46d03cf9c
ORCID for Julie M. Herniman: ORCID iD orcid.org/0000-0003-4834-1093
ORCID for G. John Langley: ORCID iD orcid.org/0000-0002-8323-7235

Catalogue record

Date deposited: 02 Sep 2016 10:24
Last modified: 07 Oct 2020 05:15

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