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Long-term antibiotic exposure in soil is associated with changes in microbial community structure and prevalence of class 1 integrons

Long-term antibiotic exposure in soil is associated with changes in microbial community structure and prevalence of class 1 integrons
Long-term antibiotic exposure in soil is associated with changes in microbial community structure and prevalence of class 1 integrons
Antimicrobial resistance is one of the most significant challenges facing the global medical community and can be attributed to the use and misuse of antibiotics. This includes use as growth promoters or for prophylaxis and treatment of bacterial infection in intensively farmed livestock from where antibiotics can enter the environment as residues in manure. We characterised the impact of the long-term application of a mixture of veterinary antibiotics alone (tylosin, sulfamethazine and chlortetracycline) on class 1 integron prevalence and soil microbiota composition. Class 1 integron prevalence increased significantly (P < 0.005) from 0.006% in control samples to 0.064% in the treated plots. Soil microbiota was analysed using 16S rRNA gene sequencing and revealed significant alterations in composition. Of the 19 significantly different (P < 0.05) OTUs identified, 16 were of the Class Proteobacteria and these decreased in abundance relative to the control plots. Only one OTU, of the Class Cyanobacteria, was shown to increase in abundance significantly; a curiosity given the established sensitivity of this class to antibiotics. We hypothesise that the overrepresentation of Proteobacteria as OTUs that decreased significantly in relative abundance, coupled with the observations of an increase in integron prevalence, may represent a strong selective pressure on these taxa.
Cleary, David W.
f4079c6d-d54b-4108-b346-b0069035bec0
Bishop, Alistair H.
4f9aec59-3ca6-4e05-9d9b-6e746f9fa2db
Zhang, Lihong
349d3c47-9aa6-4e76-baff-ff7eac5351dd
Topp, Edward
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Wellington, Elizabeth M. H.
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Gaze, William H.
4cc837d5-42a6-444f-a6ee-f75be05dbac3
Cleary, David W.
f4079c6d-d54b-4108-b346-b0069035bec0
Bishop, Alistair H.
4f9aec59-3ca6-4e05-9d9b-6e746f9fa2db
Zhang, Lihong
349d3c47-9aa6-4e76-baff-ff7eac5351dd
Topp, Edward
ac6f889b-bae7-4fcc-b4f6-7c80fa48e760
Wellington, Elizabeth M. H.
b9cdae90-6a02-49cf-aa86-cbef3267d7f6
Gaze, William H.
4cc837d5-42a6-444f-a6ee-f75be05dbac3

Cleary, David W., Bishop, Alistair H. and Zhang, Lihong et al. (2016) Long-term antibiotic exposure in soil is associated with changes in microbial community structure and prevalence of class 1 integrons. FEMS Microbiology Ecology, 92 (10), [fiw159]. (doi:10.1093/femsec/fiw159).

Record type: Article

Abstract

Antimicrobial resistance is one of the most significant challenges facing the global medical community and can be attributed to the use and misuse of antibiotics. This includes use as growth promoters or for prophylaxis and treatment of bacterial infection in intensively farmed livestock from where antibiotics can enter the environment as residues in manure. We characterised the impact of the long-term application of a mixture of veterinary antibiotics alone (tylosin, sulfamethazine and chlortetracycline) on class 1 integron prevalence and soil microbiota composition. Class 1 integron prevalence increased significantly (P < 0.005) from 0.006% in control samples to 0.064% in the treated plots. Soil microbiota was analysed using 16S rRNA gene sequencing and revealed significant alterations in composition. Of the 19 significantly different (P < 0.05) OTUs identified, 16 were of the Class Proteobacteria and these decreased in abundance relative to the control plots. Only one OTU, of the Class Cyanobacteria, was shown to increase in abundance significantly; a curiosity given the established sensitivity of this class to antibiotics. We hypothesise that the overrepresentation of Proteobacteria as OTUs that decreased significantly in relative abundance, coupled with the observations of an increase in integron prevalence, may represent a strong selective pressure on these taxa.

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Accepted/In Press date: 14 July 2016
e-pub ahead of print date: 5 August 2016
Published date: October 2016
Organisations: Faculty of Medicine

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Local EPrints ID: 400186
URI: http://eprints.soton.ac.uk/id/eprint/400186
PURE UUID: ed683c21-fbda-4274-83a9-63028691ccd5
ORCID for David W. Cleary: ORCID iD orcid.org/0000-0003-4533-0700

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Date deposited: 13 Sep 2016 08:07
Last modified: 07 Oct 2020 04:01

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Contributors

Author: David W. Cleary ORCID iD
Author: Alistair H. Bishop
Author: Lihong Zhang
Author: Edward Topp
Author: Elizabeth M. H. Wellington
Author: William H. Gaze

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