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Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400

Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400
Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400
Aerobic bacteria, such as Burkholderia xenovorans LB400, are able to degrade a wide range of polychlorobiphenyls (PCBs). Generally, these bacteria are not able to transform chlorobenzoates (CBAs), which accumulate during PCB degradation. In this study, the effects of CBAs on the growth, the morphology and the proteome of Burkholderiaxenovorans LB400 were analysed. 4-CBA and 2-CBA were observed to inhibit the growth of strain LB400 on glucose. Strain LB400 exposed to 4-CBA exhibited increased number and size of electron-dense granules in the cytoplasm, which could be polyphosphates. Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to characterise the molecular response of strain LB400 to 4-CBA. This compound induced the enzymes BenD and CatA of benzoate and catechol catabolic pathways. The induction of molecular chaperones DnaK and HtpG by 4-CBA indicated that the exposure to this compound constitutes a stressful condition for this bacterium. Additionally, the induction of some Krebs cycle enzymes was observed, probably as response to cellular energy requirements. This study contributes to the knowledge on the effects of CBA on the PCB-degrader Burkholderia xenovorans LB400.
0302-8933
289-297
Martinez, Paula
833db9ad-8cb1-4fd2-a0c6-7dc266ad0135
Aullo, Loreine
90135a87-9210-4ce0-b5f0-4c0d9dd36ac8
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Seeger, Michael
b4a0094c-c58c-4331-93f7-1e2e934bbd53
Martinez, Paula
833db9ad-8cb1-4fd2-a0c6-7dc266ad0135
Aullo, Loreine
90135a87-9210-4ce0-b5f0-4c0d9dd36ac8
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Seeger, Michael
b4a0094c-c58c-4331-93f7-1e2e934bbd53

Martinez, Paula, Aullo, Loreine, Hernandez Garcia, Marcela and Seeger, Michael (2007) Chlorobenzoate inhibits growth and induces stress proteins in the PCB-degrading bacterium Burkholderia xenovorans LB400. Archives of Microbiology, 188 (3), 289-297. (doi:10.1007/s00203-007-0247-4).

Record type: Article

Abstract

Aerobic bacteria, such as Burkholderia xenovorans LB400, are able to degrade a wide range of polychlorobiphenyls (PCBs). Generally, these bacteria are not able to transform chlorobenzoates (CBAs), which accumulate during PCB degradation. In this study, the effects of CBAs on the growth, the morphology and the proteome of Burkholderiaxenovorans LB400 were analysed. 4-CBA and 2-CBA were observed to inhibit the growth of strain LB400 on glucose. Strain LB400 exposed to 4-CBA exhibited increased number and size of electron-dense granules in the cytoplasm, which could be polyphosphates. Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to characterise the molecular response of strain LB400 to 4-CBA. This compound induced the enzymes BenD and CatA of benzoate and catechol catabolic pathways. The induction of molecular chaperones DnaK and HtpG by 4-CBA indicated that the exposure to this compound constitutes a stressful condition for this bacterium. Additionally, the induction of some Krebs cycle enzymes was observed, probably as response to cellular energy requirements. This study contributes to the knowledge on the effects of CBA on the PCB-degrader Burkholderia xenovorans LB400.

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Accepted/In Press date: 12 April 2007
e-pub ahead of print date: 24 May 2007
Published date: September 2007
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 400789
URI: http://eprints.soton.ac.uk/id/eprint/400789
ISSN: 0302-8933
PURE UUID: 0d9727c5-d294-46a9-8ab8-b73dc4a76f11

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Date deposited: 29 Sep 2016 15:35
Last modified: 14 Oct 2019 18:23

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