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Detection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil

Detection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil
Detection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil
The rhizosphere is considered as a hot-spot for gene exchange among bacteria in terrestrial ecosystems. Chilean volcanic soils are characterized by low pH and high concentrations of aluminium (Al) in the soil solution, thus Al tolerance could be important for the survival of microorganisms in these soils; loss of genes encoding for Al tolerance may affect competitiveness particularly in the rhizosphere where competition is strong. The occurrence of Al-tolerance plasmids was investigated in the rhizospheres of pasture and crop plants growing in acidic volcanic soils from southern Chile. Al tolerance plasmids were captured by biparental mating. Two types of Al tolerance plasmids could be distinguished, based on their endonuclease restriction pattern. One plasmid of each group (denoted as pRPA21 and pOPA21) was selected for further studies. The plasmids showed a high stability in presence and absence of Al. Additionally, microbial community composition in the rhizosphere soils was assessed by denaturing gradient gel electrophoresis (DGGE). Sequencing of DGGE bands revealed among others, members of the bacterial phylum Gemmatimonadetes and archaeal phylum Crenarchaeota. The present study shows that the rhizosphere of pasture and crop plants growing in Chilean volcanic soil harbors genetic mobile elements which could play a role in the adaptation of bacterial populations to environmental stressors, such as Al-toxicity.
1164-5563
255-263
Jorquera, M.A.
1d88251f-91fc-4690-9321-a1c87925c3ae
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Martinez, O.
707f0eb7-dab7-4c7c-8ff9-4ea294d1df7b
Marschner, P.
f6b413d2-9c3d-4a88-b269-086cb7748000
de la Luz Mora, M.
e97be510-2c89-4bce-82bd-da12ad01d3cd
Jorquera, M.A.
1d88251f-91fc-4690-9321-a1c87925c3ae
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Martinez, O.
707f0eb7-dab7-4c7c-8ff9-4ea294d1df7b
Marschner, P.
f6b413d2-9c3d-4a88-b269-086cb7748000
de la Luz Mora, M.
e97be510-2c89-4bce-82bd-da12ad01d3cd

Jorquera, M.A., Hernandez Garcia, Marcela, Martinez, O., Marschner, P. and de la Luz Mora, M. (2010) Detection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil. European Journal of Soil Biology, 46 (3-4), 255-263. (doi:10.1016/j.ejsobi.2010.03.005).

Record type: Article

Abstract

The rhizosphere is considered as a hot-spot for gene exchange among bacteria in terrestrial ecosystems. Chilean volcanic soils are characterized by low pH and high concentrations of aluminium (Al) in the soil solution, thus Al tolerance could be important for the survival of microorganisms in these soils; loss of genes encoding for Al tolerance may affect competitiveness particularly in the rhizosphere where competition is strong. The occurrence of Al-tolerance plasmids was investigated in the rhizospheres of pasture and crop plants growing in acidic volcanic soils from southern Chile. Al tolerance plasmids were captured by biparental mating. Two types of Al tolerance plasmids could be distinguished, based on their endonuclease restriction pattern. One plasmid of each group (denoted as pRPA21 and pOPA21) was selected for further studies. The plasmids showed a high stability in presence and absence of Al. Additionally, microbial community composition in the rhizosphere soils was assessed by denaturing gradient gel electrophoresis (DGGE). Sequencing of DGGE bands revealed among others, members of the bacterial phylum Gemmatimonadetes and archaeal phylum Crenarchaeota. The present study shows that the rhizosphere of pasture and crop plants growing in Chilean volcanic soil harbors genetic mobile elements which could play a role in the adaptation of bacterial populations to environmental stressors, such as Al-toxicity.

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Accepted/In Press date: 23 March 2010
e-pub ahead of print date: 8 April 2010
Published date: May 2010
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 400796
URI: http://eprints.soton.ac.uk/id/eprint/400796
ISSN: 1164-5563
PURE UUID: dd2b56ea-a486-4552-b629-78722633ee1f

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Date deposited: 30 Sep 2016 10:34
Last modified: 25 Nov 2019 19:49

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