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Influence of microorganisms and leaching on simazine attenuation in agricultural soils

Influence of microorganisms and leaching on simazine attenuation in agricultural soils
Influence of microorganisms and leaching on simazine attenuation in agricultural soils
Simazine is an s-triazine herbicide world widely used for the control of broadleaf weeds. The influence of leaching and microorganisms on simazine attenuation in an agricultural soil long-term treated with this herbicide was studied. To elucidate the leaching potential of simazine in this soil, undisturbed soil columns amended with simazine were placed in a specially designed system and an artificial precipitation was simulated. To evaluate the simazine removal by soil microorganisms, three soil microcosm sets were established: i) control soil; ii) soil subjected to gamma irradiation (?-soil) and iii) ?-soil inoculated with the simazine-degrading bacterium Pseudomonas sp. strain MHP41. The simazine-degrading microorganisms in soil were estimated using an indicator for respiration combined with MPN enumeration. The simazine removal in soil was monitored by GC-ECD and HPLC. In this agricultural soil the leaching of the applied simazine was negligible. The gamma irradiation decreased in more than one order of magnitude the cultivable heterotrophic bacteria and reduced the simazine-degrading microorganisms. Simazine was almost completely depleted (97%) in control soil by natural attenuation after 23 d, whereas in ?-soil only 70% of simazine was removed. The addition of the simazine-degrading strain MHP41 to ?-soil restored and upheld high stable simazine catabolic microorganisms as well as increased the simazine removal (87%). The results indicated that simazine is subjected to microbial degradation with negligible leaching in this agricultural soil and pointed out the crucial role of native microbiota in the herbicide removal.
0301-4797
S300-S305
Morgante, V.
78aa114c-78f1-4434-977f-72984b0f515d
Flores, C.
0befc617-b3cb-4905-9595-497c0e9415c5
Fadic, X.
7f212048-245b-4e07-a565-e871fbe7d842
Gonzalez, M.
d76bbd36-3155-4405-b539-b90254d4255f
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Cereceda-Balic, F.
c3d184fe-e856-4e95-8ff3-276b1dfca1aa
Seeger, M.
a5b75037-97d0-41df-a164-c8f2cc762838
Morgante, V.
78aa114c-78f1-4434-977f-72984b0f515d
Flores, C.
0befc617-b3cb-4905-9595-497c0e9415c5
Fadic, X.
7f212048-245b-4e07-a565-e871fbe7d842
Gonzalez, M.
d76bbd36-3155-4405-b539-b90254d4255f
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Cereceda-Balic, F.
c3d184fe-e856-4e95-8ff3-276b1dfca1aa
Seeger, M.
a5b75037-97d0-41df-a164-c8f2cc762838

Morgante, V., Flores, C., Fadic, X., Gonzalez, M., Hernandez Garcia, Marcela, Cereceda-Balic, F. and Seeger, M. (2012) Influence of microorganisms and leaching on simazine attenuation in agricultural soils. Journal of Environmental Management, 95, supplement Environmental Risks and Problems, Strategies to reduce them through Biotechnology and Engineering, S300-S305. (doi:10.1016/j.jenvman.2011.06.045).

Record type: Article

Abstract

Simazine is an s-triazine herbicide world widely used for the control of broadleaf weeds. The influence of leaching and microorganisms on simazine attenuation in an agricultural soil long-term treated with this herbicide was studied. To elucidate the leaching potential of simazine in this soil, undisturbed soil columns amended with simazine were placed in a specially designed system and an artificial precipitation was simulated. To evaluate the simazine removal by soil microorganisms, three soil microcosm sets were established: i) control soil; ii) soil subjected to gamma irradiation (?-soil) and iii) ?-soil inoculated with the simazine-degrading bacterium Pseudomonas sp. strain MHP41. The simazine-degrading microorganisms in soil were estimated using an indicator for respiration combined with MPN enumeration. The simazine removal in soil was monitored by GC-ECD and HPLC. In this agricultural soil the leaching of the applied simazine was negligible. The gamma irradiation decreased in more than one order of magnitude the cultivable heterotrophic bacteria and reduced the simazine-degrading microorganisms. Simazine was almost completely depleted (97%) in control soil by natural attenuation after 23 d, whereas in ?-soil only 70% of simazine was removed. The addition of the simazine-degrading strain MHP41 to ?-soil restored and upheld high stable simazine catabolic microorganisms as well as increased the simazine removal (87%). The results indicated that simazine is subjected to microbial degradation with negligible leaching in this agricultural soil and pointed out the crucial role of native microbiota in the herbicide removal.

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Accepted/In Press date: 21 June 2011
e-pub ahead of print date: 28 July 2011
Published date: March 2012
Organisations: Centre for Biological Sciences

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Local EPrints ID: 400799
URI: http://eprints.soton.ac.uk/id/eprint/400799
ISSN: 0301-4797
PURE UUID: af69c865-0bff-4a7e-8b86-941ed26fbd86

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Date deposited: 30 Sep 2016 10:48
Last modified: 15 Mar 2024 02:29

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Contributors

Author: V. Morgante
Author: C. Flores
Author: X. Fadic
Author: M. Gonzalez
Author: Marcela Hernandez Garcia
Author: F. Cereceda-Balic
Author: M. Seeger

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