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Changes in bacterial communities by post-emergent herbicides in an Andisol fertilized with urea as revealed by DGGE

Changes in bacterial communities by post-emergent herbicides in an Andisol fertilized with urea as revealed by DGGE
Changes in bacterial communities by post-emergent herbicides in an Andisol fertilized with urea as revealed by DGGE
Large amounts of urea and various herbicides are applied to Chilean Andisols by farmers; however, how different herbicide types affect bacterial communities in urea-fertilized soils remains unknown. In this study, the effect of post-emergent herbicides (MCPA, flumetsulam, fluroxypyr, triclopyr, clopyralid and picloram) on total bacterial (TB) and ammonia-oxidizing bacteria (AOB) communities was investigated in a urea-fertilized soil microcosm using denaturing gradient gel electrophoresis at days 1 and 15 of incubation. The residual concentrations of herbicides in microcosm soils were measured by high-performance liquid chromatography. Differences in bacterial communities were analyzed and visualized with non-metric multidimensional scaling. All of the tested herbicides decreased in microcosm soils, reaching 46–98% of dissipation at 15 days. Changes in the TB and AOB communities were variable, depending on the urea and herbicide dosage, herbicide type and sampling time. Under the recommended dose of urea (570 mg kg?1 soil), soils that were treated with MCPA and triclopyr showed significant changes in TB and AOB community structures compared to control soils at both sampling times. When a 2-fold-higher recommended dose of urea (1140 mg kg?1 soil) was applied, the TB communities were mainly affected by flumetsulam and picloram at both sampling times. Changes in the AOB communities were mainly observed in soils that were treated with MCPA and fluroxypyr under both urea doses. This study determined which herbicides (and doses) significantly affect the bacterial communities in urea-fertilized soils. This evidence can be very useful when management practices are being designed, revised and/or established in Andisols.
0929-1393
141-151
Marileo, Luis
56b0782b-9e33-4879-b58f-eb2e67c9f739
Jorquera, Milko
d741ebb3-6512-4644-ac7c-a910d323bb71
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Briceño, Gabriela
12a97934-9ac8-4ec2-846f-e4525521e14c
Mora, Maria de la Luz
43793158-0f7a-462e-89e9-288eb421b982
Demanet, Rolando
a504b4d6-0a88-4ce2-afe3-478e4b99dc2f
Palma, Gabriela
96c5e517-3ce0-45e2-b03e-3d4954f6981c
Marileo, Luis
56b0782b-9e33-4879-b58f-eb2e67c9f739
Jorquera, Milko
d741ebb3-6512-4644-ac7c-a910d323bb71
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Briceño, Gabriela
12a97934-9ac8-4ec2-846f-e4525521e14c
Mora, Maria de la Luz
43793158-0f7a-462e-89e9-288eb421b982
Demanet, Rolando
a504b4d6-0a88-4ce2-afe3-478e4b99dc2f
Palma, Gabriela
96c5e517-3ce0-45e2-b03e-3d4954f6981c

Marileo, Luis, Jorquera, Milko, Hernandez Garcia, Marcela, Briceño, Gabriela, Mora, Maria de la Luz, Demanet, Rolando and Palma, Gabriela (2016) Changes in bacterial communities by post-emergent herbicides in an Andisol fertilized with urea as revealed by DGGE. Applied Soil Ecology, 101, 141-151. (doi:10.1016/j.apsoil.2016.02.003).

Record type: Article

Abstract

Large amounts of urea and various herbicides are applied to Chilean Andisols by farmers; however, how different herbicide types affect bacterial communities in urea-fertilized soils remains unknown. In this study, the effect of post-emergent herbicides (MCPA, flumetsulam, fluroxypyr, triclopyr, clopyralid and picloram) on total bacterial (TB) and ammonia-oxidizing bacteria (AOB) communities was investigated in a urea-fertilized soil microcosm using denaturing gradient gel electrophoresis at days 1 and 15 of incubation. The residual concentrations of herbicides in microcosm soils were measured by high-performance liquid chromatography. Differences in bacterial communities were analyzed and visualized with non-metric multidimensional scaling. All of the tested herbicides decreased in microcosm soils, reaching 46–98% of dissipation at 15 days. Changes in the TB and AOB communities were variable, depending on the urea and herbicide dosage, herbicide type and sampling time. Under the recommended dose of urea (570 mg kg?1 soil), soils that were treated with MCPA and triclopyr showed significant changes in TB and AOB community structures compared to control soils at both sampling times. When a 2-fold-higher recommended dose of urea (1140 mg kg?1 soil) was applied, the TB communities were mainly affected by flumetsulam and picloram at both sampling times. Changes in the AOB communities were mainly observed in soils that were treated with MCPA and fluroxypyr under both urea doses. This study determined which herbicides (and doses) significantly affect the bacterial communities in urea-fertilized soils. This evidence can be very useful when management practices are being designed, revised and/or established in Andisols.

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More information

Accepted/In Press date: 3 February 2016
e-pub ahead of print date: 12 February 2016
Published date: May 2016
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 400804
URI: http://eprints.soton.ac.uk/id/eprint/400804
ISSN: 0929-1393
PURE UUID: ba01e836-6b57-4916-ad58-366de45f7984

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Date deposited: 27 Sep 2016 11:07
Last modified: 16 Dec 2019 19:42

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