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Transcriptional profiling of Th2 cells identifies pathogenic features associated with asthma

Transcriptional profiling of Th2 cells identifies pathogenic features associated with asthma
Transcriptional profiling of Th2 cells identifies pathogenic features associated with asthma
Allergic asthma and rhinitis are two common chronic allergic diseases that affect the lungs and nose, respectively. Both diseases share clinical and pathological features characteristic of excessive allergen-induced type 2 inflammation, orchestrated by memory CD4+ T cells that produce type 2 cytokines (Th2 cells). However, a large majority of subjects with allergic rhinitis do not develop asthma, suggesting divergence in disease mechanisms. Because Th2 cells play a pathogenic role in both these diseases and are also present in healthy nonallergic subjects, we performed global transcriptional profiling to determine whether there are qualitative differences in Th2 cells from subjects with allergic asthma, rhinitis, and healthy controls. Th2 cells from asthmatic subjects expressed higher levels of several genes that promote their survival as well as alter their metabolic pathways to favor persistence at sites of allergic inflammation. In addition, genes that enhanced Th2 polarization and Th2 cytokine production were also upregulated in asthma. Several genes that oppose T cell activation were downregulated in asthma, suggesting enhanced activation potential of Th2 cells from asthmatic subjects. Many novel genes with poorly defined functions were also differentially expressed in asthma. Thus, our transcriptomic analysis of circulating Th2 cells has identified several molecules that are likely to confer pathogenic features to Th2 cells that are either unique or common to both asthma and rhinitis
0022-1767
655-664
Seumois, G.
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Zapardiel-Gonzalo, J.
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White, B.
e1ed7af7-d5ea-45ef-b2f5-2c95a83842c4
Singh, D.
150efb02-377d-4ead-ab19-3606dbc7c00d
Schulten, V.
16262062-1ab5-4b07-87bd-4d40a3e335e5
Dillon, M.
38fc4268-0796-4a16-80dc-58bb17721752
Hinz, D.
84c32e51-1486-48ba-a9c2-c19d77eaf9a5
Broide, D. H.
a69fe3a9-97cb-4a99-b671-a80bc592fe54
Sette, A.
757a9b9b-8e73-4a7d-ab65-97949fa65d8a
Peters, B.
044a12e3-64de-49b0-82f1-dfda5233a35e
Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1
Seumois, G.
fa404dbb-42e3-47a2-9479-cc5c34c61086
Zapardiel-Gonzalo, J.
c5cfc7c0-7ab0-4409-9274-f99540dff71c
White, B.
e1ed7af7-d5ea-45ef-b2f5-2c95a83842c4
Singh, D.
150efb02-377d-4ead-ab19-3606dbc7c00d
Schulten, V.
16262062-1ab5-4b07-87bd-4d40a3e335e5
Dillon, M.
38fc4268-0796-4a16-80dc-58bb17721752
Hinz, D.
84c32e51-1486-48ba-a9c2-c19d77eaf9a5
Broide, D. H.
a69fe3a9-97cb-4a99-b671-a80bc592fe54
Sette, A.
757a9b9b-8e73-4a7d-ab65-97949fa65d8a
Peters, B.
044a12e3-64de-49b0-82f1-dfda5233a35e
Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1

Seumois, G., Zapardiel-Gonzalo, J., White, B., Singh, D., Schulten, V., Dillon, M., Hinz, D., Broide, D. H., Sette, A., Peters, B. and Vijayanand, Pandurangan (2016) Transcriptional profiling of Th2 cells identifies pathogenic features associated with asthma. Journal of Immunology, 197 (2), 655-664. (doi:10.4049/jimmunol.1600397).

Record type: Article

Abstract

Allergic asthma and rhinitis are two common chronic allergic diseases that affect the lungs and nose, respectively. Both diseases share clinical and pathological features characteristic of excessive allergen-induced type 2 inflammation, orchestrated by memory CD4+ T cells that produce type 2 cytokines (Th2 cells). However, a large majority of subjects with allergic rhinitis do not develop asthma, suggesting divergence in disease mechanisms. Because Th2 cells play a pathogenic role in both these diseases and are also present in healthy nonallergic subjects, we performed global transcriptional profiling to determine whether there are qualitative differences in Th2 cells from subjects with allergic asthma, rhinitis, and healthy controls. Th2 cells from asthmatic subjects expressed higher levels of several genes that promote their survival as well as alter their metabolic pathways to favor persistence at sites of allergic inflammation. In addition, genes that enhanced Th2 polarization and Th2 cytokine production were also upregulated in asthma. Several genes that oppose T cell activation were downregulated in asthma, suggesting enhanced activation potential of Th2 cells from asthmatic subjects. Many novel genes with poorly defined functions were also differentially expressed in asthma. Thus, our transcriptomic analysis of circulating Th2 cells has identified several molecules that are likely to confer pathogenic features to Th2 cells that are either unique or common to both asthma and rhinitis

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Accepted/In Press date: 13 May 2016
e-pub ahead of print date: 5 July 2016
Published date: 15 July 2016
Organisations: NIHR Southampton Respiratory Biomedical Research Unit, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 400962
URI: https://eprints.soton.ac.uk/id/eprint/400962
ISSN: 0022-1767
PURE UUID: d3334a5c-00d8-4f8b-b7c8-3b19e88dfb99

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Date deposited: 30 Sep 2016 12:47
Last modified: 09 Jan 2018 17:50

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