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Analysis of sequence variability in the CART gene in relation to obesity in a Caucasian population

Analysis of sequence variability in the CART gene in relation to obesity in a Caucasian population
Analysis of sequence variability in the CART gene in relation to obesity in a Caucasian population
Background

Cocaine and amphetamine regulated transcript (CART) is an anorectic neuropeptide located principally in hypothalamus. CART has been shown to be involved in control of feeding behavior, but a direct relationship with obesity has not been established. The aim of this study was to evaluate the effect of polymorphisms within the CART gene with regards to a possible association with obesity in a Caucasian population.

Results

Screening of the entire gene as well as a 3.7 kb region of 5' upstream sequence revealed 31 SNPs and 3 rare variants ; 14 of which were subsequently genotyped in 292 French morbidly obese subjects and 368 controls. Haplotype analysis suggested an association with obesity which was found to be mainly due to SNP-3608T>C (rs7379701) (p = 0.009). Genotyping additional cases and controls also of European Caucasian origin supported further this possible association between the CART SNP -3608T>C T allele and obesity (global p-value = 0.0005). Functional studies also suggested that the SNP -3608T>C could modulate nuclear protein binding.

Conclusion

CART SNP -3608T>C may possibly contribute to the genetic risk for obesity in the Caucasian population. However confirmation of the importance of the role of the CART gene in energy homeostasis and obesity will require investigation and replication in further populations.
1471-2156
1-12
Guérardel, Audrey
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Barat-Houari, Mouna
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Vasseur, Francis
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Dina, Christian
3df025f2-1369-4ea6-84a3-f58994951f68
Vatin, Vincent
fab78f9f-5fe5-432c-9c87-c88326520b0f
Clément, Karine
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Eberlé, Delphine
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Vasseur-Delannoy, Valérie
9d0569f3-6f76-465f-af6f-99b2d1463a8e
Bell, Christopher G.
44982df7-0746-4cdb-bed1-0bdfe68f1a64
Galan, Pilar
351ddba8-bd81-45d5-8c9e-bdc9f8e81287
Hercberg, Serge
9de31452-a0ff-4918-ae84-666b71056a41
Helbecque, Nicole
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Potoczna, Natascha
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Horber, Fritz F.
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Boutin, Philippe
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Froguel, Philippe
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Guérardel, Audrey
3a267c65-7cb7-46f7-a0e2-7f3bbbddc11e
Barat-Houari, Mouna
85ac0a7f-4d2b-4e46-aefa-4d4f309a2697
Vasseur, Francis
1a060d54-faf0-42a0-a5a1-5ca733863fd8
Dina, Christian
3df025f2-1369-4ea6-84a3-f58994951f68
Vatin, Vincent
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Clément, Karine
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Eberlé, Delphine
d2dc138f-baae-4fba-815f-91bc52c32dd4
Vasseur-Delannoy, Valérie
9d0569f3-6f76-465f-af6f-99b2d1463a8e
Bell, Christopher G.
44982df7-0746-4cdb-bed1-0bdfe68f1a64
Galan, Pilar
351ddba8-bd81-45d5-8c9e-bdc9f8e81287
Hercberg, Serge
9de31452-a0ff-4918-ae84-666b71056a41
Helbecque, Nicole
865995d5-94b5-4131-85ce-30a9b5823760
Potoczna, Natascha
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Horber, Fritz F.
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Boutin, Philippe
f0998caa-ad41-4cd5-ad8f-0878cc6b0378
Froguel, Philippe
563ee961-98a3-4aed-98a5-d70e8350ef8c

Guérardel, Audrey, Barat-Houari, Mouna, Vasseur, Francis, Dina, Christian, Vatin, Vincent, Clément, Karine, Eberlé, Delphine, Vasseur-Delannoy, Valérie, Bell, Christopher G., Galan, Pilar, Hercberg, Serge, Helbecque, Nicole, Potoczna, Natascha, Horber, Fritz F., Boutin, Philippe and Froguel, Philippe (2005) Analysis of sequence variability in the CART gene in relation to obesity in a Caucasian population. BMC Genetics, 6 (19), 1-12. (doi:10.1186/1471-2156-6-19). (PMID:12654232)

Record type: Article

Abstract

Background

Cocaine and amphetamine regulated transcript (CART) is an anorectic neuropeptide located principally in hypothalamus. CART has been shown to be involved in control of feeding behavior, but a direct relationship with obesity has not been established. The aim of this study was to evaluate the effect of polymorphisms within the CART gene with regards to a possible association with obesity in a Caucasian population.

Results

Screening of the entire gene as well as a 3.7 kb region of 5' upstream sequence revealed 31 SNPs and 3 rare variants ; 14 of which were subsequently genotyped in 292 French morbidly obese subjects and 368 controls. Haplotype analysis suggested an association with obesity which was found to be mainly due to SNP-3608T>C (rs7379701) (p = 0.009). Genotyping additional cases and controls also of European Caucasian origin supported further this possible association between the CART SNP -3608T>C T allele and obesity (global p-value = 0.0005). Functional studies also suggested that the SNP -3608T>C could modulate nuclear protein binding.

Conclusion

CART SNP -3608T>C may possibly contribute to the genetic risk for obesity in the Caucasian population. However confirmation of the importance of the role of the CART gene in energy homeostasis and obesity will require investigation and replication in further populations.

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More information

Accepted/In Press date: 11 April 2005
Published date: 11 April 2005
Organisations: Human Development & Health, Centre for Biological Sciences, MRC Life-Course Epidemiology Unit

Identifiers

Local EPrints ID: 400999
URI: http://eprints.soton.ac.uk/id/eprint/400999
ISSN: 1471-2156
PURE UUID: a286a250-87fe-4ebd-ab04-7ac298d6415f
ORCID for Christopher G. Bell: ORCID iD orcid.org/0000-0003-4601-1242

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Date deposited: 04 Oct 2016 15:58
Last modified: 17 Dec 2019 01:33

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Contributors

Author: Audrey Guérardel
Author: Mouna Barat-Houari
Author: Francis Vasseur
Author: Christian Dina
Author: Vincent Vatin
Author: Karine Clément
Author: Delphine Eberlé
Author: Valérie Vasseur-Delannoy
Author: Christopher G. Bell ORCID iD
Author: Pilar Galan
Author: Serge Hercberg
Author: Nicole Helbecque
Author: Natascha Potoczna
Author: Fritz F. Horber
Author: Philippe Boutin
Author: Philippe Froguel

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