The University of Southampton
University of Southampton Institutional Repository

Development of microfluidic pre-concentration system for metals in seawater

Development of microfluidic pre-concentration system for metals in seawater
Development of microfluidic pre-concentration system for metals in seawater
In-situ marine biogeochemical sensing allows measurement at high frequency to investigate short and long-term variability in processes in ocean waters. In trace analyses, not only the low concentrations of analytes but also possible interferences from the matrix and other elements must be considered, therefore a pre-concentration step prior to the determination of trace metals needs to be implemented. The way forward for improved in-situ analyses of manganese in seawater was to build on the existing technology of the Sensors Group at NOC, Southampton and develop a pre-concentration system for coupling to spectrophotometric Lab on a Chip (LOAC) technologies.

This thesis describes the development and optimisation of LOAC analyser for manganese determination in aquatic environments, integrated with pre-concentration. Optimised conditions for the extraction of manganese from seawater with a Toyopearl iminodiacetate resin column were developed using Mn-54 tracer. Conditions evaluated were optimal pH for manganese removal, optimal flow rate required for full recovery of manganese, and the concentration and the volume of the acid solution needed for quantitative elution of manganese from the resin.

The LOAC system used the colorimetric 1-(2-Pyridylazo)-2-naphthol (PAN) method for Mn determinations. The modified method was used for manganese determinations, when sodium dodecyl sulfate (SDS) was used as surfactant, with calibration curves constructed and precision (in the range 1.08 - 3.81 % RSD) and the LOD of the method assessed (3SD, typically 14 nM with 10 cm cell).

Results of the adaptation of the modified PAN method on the chip and its coupling to a resin column for collection of manganese proves the concept of on-chip pre-concentration.

The design and production method of the microfluidic chips and the operation procedure of the bench-top LOAC manganese determination system with the resin column implemented on the chip are described. The LOAC system coupled with the resin column and PAN chemistry was used for manganese determination with calibration curve constructed and precision of the method determined (0.86 - 2.69 % RSD).
Skiba, Marta
dfa909a9-5234-4de5-8465-03b464a87fec
Skiba, Marta
dfa909a9-5234-4de5-8465-03b464a87fec
Statham, Peter
51458f15-d6e2-4231-8bba-d0567f9e440c

(2015) Development of microfluidic pre-concentration system for metals in seawater. University of Southampton, Ocean & Earth Science, Doctoral Thesis, 192pp.

Record type: Thesis (Doctoral)

Abstract

In-situ marine biogeochemical sensing allows measurement at high frequency to investigate short and long-term variability in processes in ocean waters. In trace analyses, not only the low concentrations of analytes but also possible interferences from the matrix and other elements must be considered, therefore a pre-concentration step prior to the determination of trace metals needs to be implemented. The way forward for improved in-situ analyses of manganese in seawater was to build on the existing technology of the Sensors Group at NOC, Southampton and develop a pre-concentration system for coupling to spectrophotometric Lab on a Chip (LOAC) technologies.

This thesis describes the development and optimisation of LOAC analyser for manganese determination in aquatic environments, integrated with pre-concentration. Optimised conditions for the extraction of manganese from seawater with a Toyopearl iminodiacetate resin column were developed using Mn-54 tracer. Conditions evaluated were optimal pH for manganese removal, optimal flow rate required for full recovery of manganese, and the concentration and the volume of the acid solution needed for quantitative elution of manganese from the resin.

The LOAC system used the colorimetric 1-(2-Pyridylazo)-2-naphthol (PAN) method for Mn determinations. The modified method was used for manganese determinations, when sodium dodecyl sulfate (SDS) was used as surfactant, with calibration curves constructed and precision (in the range 1.08 - 3.81 % RSD) and the LOD of the method assessed (3SD, typically 14 nM with 10 cm cell).

Results of the adaptation of the modified PAN method on the chip and its coupling to a resin column for collection of manganese proves the concept of on-chip pre-concentration.

The design and production method of the microfluidic chips and the operation procedure of the bench-top LOAC manganese determination system with the resin column implemented on the chip are described. The LOAC system coupled with the resin column and PAN chemistry was used for manganese determination with calibration curve constructed and precision of the method determined (0.86 - 2.69 % RSD).

PDF
Skiba, Marta_PhD Thesis_2016.pdf - Other
Download (3MB)

More information

Published date: 15 October 2015
Organisations: University of Southampton, Ocean and Earth Science

Identifiers

Local EPrints ID: 401162
URI: http://eprints.soton.ac.uk/id/eprint/401162
PURE UUID: 7a10f7ac-68cc-44ce-a11b-56b94ca6fa63

Catalogue record

Date deposited: 12 Oct 2016 12:29
Last modified: 17 Jul 2017 18:04

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×