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Intracellular production of cyclic peptide libraries with SICLOPPS

Intracellular production of cyclic peptide libraries with SICLOPPS
Intracellular production of cyclic peptide libraries with SICLOPPS
Cyclic peptides are an important class of molecules that are increasingly viewed as an ideal scaffold for inhibition of protein-protein interactions (PPI). Here we detail an approach that enables the intracellular synthesis of cyclic peptide libraries of around 108 members. The method utilizes split intein mediated circular ligation of peptides and proteins (SICLOPPS), taking advantage of split intein splicing to cyclize a library of peptide sequences. SICLOPPS allows the ring size, set residues and number of random residues within a library to be predetermined by the user. SICLOPPS libraries have been combined with a variety of cell-based screens to identify cyclic peptide inhibitors of a variety of enzymes and protein-protein interactions.
1495
27-39
Springer
Osher, Eliot
bcaf2f72-05de-41b2-aa0d-995f4c66d8f0
Tavassoli, Ali
d561cf8f-2669-46b5-b6e1-2016c85d63b2
Mootz, Henning D.
Osher, Eliot
bcaf2f72-05de-41b2-aa0d-995f4c66d8f0
Tavassoli, Ali
d561cf8f-2669-46b5-b6e1-2016c85d63b2
Mootz, Henning D.

Osher, Eliot and Tavassoli, Ali (2017) Intracellular production of cyclic peptide libraries with SICLOPPS. In, Mootz, Henning D. (ed.) Split Inteins. (Methods in Molecular Biology, 1495) New York, US. Springer, pp. 27-39. (doi:10.1007/978-1-4939-6451-2_3).

Record type: Book Section

Abstract

Cyclic peptides are an important class of molecules that are increasingly viewed as an ideal scaffold for inhibition of protein-protein interactions (PPI). Here we detail an approach that enables the intracellular synthesis of cyclic peptide libraries of around 108 members. The method utilizes split intein mediated circular ligation of peptides and proteins (SICLOPPS), taking advantage of split intein splicing to cyclize a library of peptide sequences. SICLOPPS allows the ring size, set residues and number of random residues within a library to be predetermined by the user. SICLOPPS libraries have been combined with a variety of cell-based screens to identify cyclic peptide inhibitors of a variety of enzymes and protein-protein interactions.

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e-pub ahead of print date: 7 October 2016
Published date: 2017

Identifiers

Local EPrints ID: 401529
URI: http://eprints.soton.ac.uk/id/eprint/401529
PURE UUID: 25d96988-1739-45bd-9729-5c0e6321346b
ORCID for Ali Tavassoli: ORCID iD orcid.org/0000-0002-7420-5063

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Date deposited: 18 Oct 2016 11:03
Last modified: 15 Mar 2024 03:26

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Contributors

Author: Eliot Osher
Author: Ali Tavassoli ORCID iD
Editor: Henning D. Mootz

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