Sex specific vitamin D effects on blood coagulation among overweight adults
Sex specific vitamin D effects on blood coagulation among overweight adults
BACKGROUND: Overweight adults are at increased risk for cardiovascular disease and vitamin D deficiency, whereas an important feature to vitamin D physiology is its sex dependence. The aim of this study was to examine whether vitamin D status improvement exerts a sexually dimorphic effect on serum proteins associated with cardiovascular risk among overweight adults.
METHODS: Unprocessed serum from age- and BMI-matched men (n=26) and pre-menopausal women (n=24) with vitamin D deficiency and after they achieved sufficiency through a 12-month nutritional intervention was analyzed using our previously published depletion-free, quantitative proteomics method. Key findings were verified with ELISA. Differentially expressed proteins were subjected to in silico bioinformatics assessment using Principal Component Analysis, hierarchical clustering and Metacore™ pathway analysis. All mass spectrometry proteomic data are available via ProteomeXchange (identifier: PXD003663).
RESULTS: A total of 282 proteins were differentially expressed after the intervention between men and women (p-value ? 0.05), in which the blood coagulation pathway was significantly enriched. In agreement with the proteomics findings, ELISA measurements showed vitamin-K dependent protein C, von Willebrand factor, fibrinogen gamma chain and multimerin-1 proteins, of relevance to blood coagulation, to be differentially affected (p-value ? 0.05) between sexes after vitamin D status correction.
CONCLUSIONS: This study identified novel protein-level molecular indicators on the sexually dimorphic effect of vitamin D status correction associated with blood coagulation among overweight adults. These sex-mediated vitamin D effects should be factored in the design and interpretation of vitamin D observational and interventional studies testing cardiometabolic outcomes.
1-22
Al-Daghri, Nasser M.
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Alokail, Majed S.
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Manousopoulou, Antigoni
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Heinson, Ashley
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Al-Attas, Oman
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Al-Saleh, Yousef
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Sabico, Shaun
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Yakout, Sobhy
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Woelk, Christopher H.
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Chrousos, Geroge P.
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Garbis, Spiros D.
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Al-Daghri, Nasser M.
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Alokail, Majed S.
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Manousopoulou, Antigoni
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Heinson, Ashley
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Al-Attas, Oman
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Al-Saleh, Yousef
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Sabico, Shaun
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Yakout, Sobhy
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Woelk, Christopher H.
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Chrousos, Geroge P.
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Garbis, Spiros D.
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Al-Daghri, Nasser M., Alokail, Majed S., Manousopoulou, Antigoni, Heinson, Ashley, Al-Attas, Oman, Al-Saleh, Yousef, Sabico, Shaun, Yakout, Sobhy, Woelk, Christopher H., Chrousos, Geroge P. and Garbis, Spiros D.
(2016)
Sex specific vitamin D effects on blood coagulation among overweight adults.
European Journal of Clinical Investigation, .
(doi:10.1111/eci.12688).
(PMID:27727459)
Abstract
BACKGROUND: Overweight adults are at increased risk for cardiovascular disease and vitamin D deficiency, whereas an important feature to vitamin D physiology is its sex dependence. The aim of this study was to examine whether vitamin D status improvement exerts a sexually dimorphic effect on serum proteins associated with cardiovascular risk among overweight adults.
METHODS: Unprocessed serum from age- and BMI-matched men (n=26) and pre-menopausal women (n=24) with vitamin D deficiency and after they achieved sufficiency through a 12-month nutritional intervention was analyzed using our previously published depletion-free, quantitative proteomics method. Key findings were verified with ELISA. Differentially expressed proteins were subjected to in silico bioinformatics assessment using Principal Component Analysis, hierarchical clustering and Metacore™ pathway analysis. All mass spectrometry proteomic data are available via ProteomeXchange (identifier: PXD003663).
RESULTS: A total of 282 proteins were differentially expressed after the intervention between men and women (p-value ? 0.05), in which the blood coagulation pathway was significantly enriched. In agreement with the proteomics findings, ELISA measurements showed vitamin-K dependent protein C, von Willebrand factor, fibrinogen gamma chain and multimerin-1 proteins, of relevance to blood coagulation, to be differentially affected (p-value ? 0.05) between sexes after vitamin D status correction.
CONCLUSIONS: This study identified novel protein-level molecular indicators on the sexually dimorphic effect of vitamin D status correction associated with blood coagulation among overweight adults. These sex-mediated vitamin D effects should be factored in the design and interpretation of vitamin D observational and interventional studies testing cardiometabolic outcomes.
Text
Al-Daghri_et_al-Sex specific vitamin D effects on blood coagulation among overweight adults_2016-European_Journal_of_Clinical_Investigation.pdf
- Accepted Manuscript
More information
Accepted/In Press date: 6 October 2016
e-pub ahead of print date: 11 October 2016
Organisations:
Faculty of Medicine
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Local EPrints ID: 401544
URI: http://eprints.soton.ac.uk/id/eprint/401544
ISSN: 0014-2972
PURE UUID: 1b9dc2e7-82a6-44d4-8d69-8f189382e267
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Date deposited: 18 Oct 2016 12:48
Last modified: 15 Mar 2024 05:58
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Contributors
Author:
Nasser M. Al-Daghri
Author:
Majed S. Alokail
Author:
Antigoni Manousopoulou
Author:
Ashley Heinson
Author:
Oman Al-Attas
Author:
Yousef Al-Saleh
Author:
Shaun Sabico
Author:
Sobhy Yakout
Author:
Christopher H. Woelk
Author:
Geroge P. Chrousos
Author:
Spiros D. Garbis
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