The University of Southampton
University of Southampton Institutional Repository

Sex specific vitamin D effects on blood coagulation among overweight adults

Sex specific vitamin D effects on blood coagulation among overweight adults
Sex specific vitamin D effects on blood coagulation among overweight adults
BACKGROUND: Overweight adults are at increased risk for cardiovascular disease and vitamin D deficiency, whereas an important feature to vitamin D physiology is its sex dependence. The aim of this study was to examine whether vitamin D status improvement exerts a sexually dimorphic effect on serum proteins associated with cardiovascular risk among overweight adults.

METHODS: Unprocessed serum from age- and BMI-matched men (n=26) and pre-menopausal women (n=24) with vitamin D deficiency and after they achieved sufficiency through a 12-month nutritional intervention was analyzed using our previously published depletion-free, quantitative proteomics method. Key findings were verified with ELISA. Differentially expressed proteins were subjected to in silico bioinformatics assessment using Principal Component Analysis, hierarchical clustering and Metacore™ pathway analysis. All mass spectrometry proteomic data are available via ProteomeXchange (identifier: PXD003663).

RESULTS: A total of 282 proteins were differentially expressed after the intervention between men and women (p-value ? 0.05), in which the blood coagulation pathway was significantly enriched. In agreement with the proteomics findings, ELISA measurements showed vitamin-K dependent protein C, von Willebrand factor, fibrinogen gamma chain and multimerin-1 proteins, of relevance to blood coagulation, to be differentially affected (p-value ? 0.05) between sexes after vitamin D status correction.

CONCLUSIONS: This study identified novel protein-level molecular indicators on the sexually dimorphic effect of vitamin D status correction associated with blood coagulation among overweight adults. These sex-mediated vitamin D effects should be factored in the design and interpretation of vitamin D observational and interventional studies testing cardiometabolic outcomes. This article is protected by copyright. All rights reserved.
0014-2972
1-22
Al-Daghri, Nasser M.
0bf1023c-a104-4f74-8b06-87780dfbd8b4
Alokail, Majed S.
9adf2d61-d9f7-4095-bc36-701f0680646a
Manousopoulou, Antigoni
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Heinson, Ashley
7724186b-dbc6-4825-9bb2-6be29e30e18e
Al-Attas, Oman
0fd3616f-cac5-4d55-8b79-9b5ec2db8db5
Al-Saleh, Yousef
b6a30c8e-441b-418f-9c77-cf61799fd2b1
Sabico, Shaun
dd062ab8-a57c-4b27-a35c-b2d0d6b6b0f8
Yakout, Sobhy
59a1ec84-40df-429c-8fc1-53febf12d89b
Woelk, Christopher H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Chrousos, Geroge P.
ce5c46b0-b769-4594-b2e6-98dafd9f8996
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Al-Daghri, Nasser M.
0bf1023c-a104-4f74-8b06-87780dfbd8b4
Alokail, Majed S.
9adf2d61-d9f7-4095-bc36-701f0680646a
Manousopoulou, Antigoni
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Heinson, Ashley
7724186b-dbc6-4825-9bb2-6be29e30e18e
Al-Attas, Oman
0fd3616f-cac5-4d55-8b79-9b5ec2db8db5
Al-Saleh, Yousef
b6a30c8e-441b-418f-9c77-cf61799fd2b1
Sabico, Shaun
dd062ab8-a57c-4b27-a35c-b2d0d6b6b0f8
Yakout, Sobhy
59a1ec84-40df-429c-8fc1-53febf12d89b
Woelk, Christopher H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Chrousos, Geroge P.
ce5c46b0-b769-4594-b2e6-98dafd9f8996
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd

Al-Daghri, Nasser M., Alokail, Majed S., Manousopoulou, Antigoni, Heinson, Ashley, Al-Attas, Oman, Al-Saleh, Yousef, Sabico, Shaun, Yakout, Sobhy, Woelk, Christopher H., Chrousos, Geroge P. and Garbis, Spiros D. (2016) Sex specific vitamin D effects on blood coagulation among overweight adults. European Journal of Clinical Investigation, 1-22. (doi:10.1111/eci.12688). (PMID:27727459)

Record type: Article

Abstract

BACKGROUND: Overweight adults are at increased risk for cardiovascular disease and vitamin D deficiency, whereas an important feature to vitamin D physiology is its sex dependence. The aim of this study was to examine whether vitamin D status improvement exerts a sexually dimorphic effect on serum proteins associated with cardiovascular risk among overweight adults.

METHODS: Unprocessed serum from age- and BMI-matched men (n=26) and pre-menopausal women (n=24) with vitamin D deficiency and after they achieved sufficiency through a 12-month nutritional intervention was analyzed using our previously published depletion-free, quantitative proteomics method. Key findings were verified with ELISA. Differentially expressed proteins were subjected to in silico bioinformatics assessment using Principal Component Analysis, hierarchical clustering and Metacore™ pathway analysis. All mass spectrometry proteomic data are available via ProteomeXchange (identifier: PXD003663).

RESULTS: A total of 282 proteins were differentially expressed after the intervention between men and women (p-value ? 0.05), in which the blood coagulation pathway was significantly enriched. In agreement with the proteomics findings, ELISA measurements showed vitamin-K dependent protein C, von Willebrand factor, fibrinogen gamma chain and multimerin-1 proteins, of relevance to blood coagulation, to be differentially affected (p-value ? 0.05) between sexes after vitamin D status correction.

CONCLUSIONS: This study identified novel protein-level molecular indicators on the sexually dimorphic effect of vitamin D status correction associated with blood coagulation among overweight adults. These sex-mediated vitamin D effects should be factored in the design and interpretation of vitamin D observational and interventional studies testing cardiometabolic outcomes. This article is protected by copyright. All rights reserved.

Text
Al-Daghri_et_al-Sex specific vitamin D effects on blood coagulation among overweight adults_2016-European_Journal_of_Clinical_Investigation.pdf - Accepted Manuscript
Download (546kB)

More information

Accepted/In Press date: 6 October 2016
e-pub ahead of print date: 11 October 2016
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 401544
URI: http://eprints.soton.ac.uk/id/eprint/401544
ISSN: 0014-2972
PURE UUID: 1b9dc2e7-82a6-44d4-8d69-8f189382e267
ORCID for Spiros D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805

Catalogue record

Date deposited: 18 Oct 2016 12:48
Last modified: 10 Dec 2019 06:22

Export record

Altmetrics

Contributors

Author: Nasser M. Al-Daghri
Author: Majed S. Alokail
Author: Antigoni Manousopoulou
Author: Ashley Heinson
Author: Oman Al-Attas
Author: Yousef Al-Saleh
Author: Shaun Sabico
Author: Sobhy Yakout
Author: Geroge P. Chrousos

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×