The University of Southampton
University of Southampton Institutional Repository

Singlet oxygen initiates a plastid signal controlling photosynthetic gene expression

Singlet oxygen initiates a plastid signal controlling photosynthetic gene expression
Singlet oxygen initiates a plastid signal controlling photosynthetic gene expression
Retrograde signals from the plastid regulate photosynthesis-associated nuclear genes and are essential to successful chloroplast biogenesis. One model is that a positive haem-related signal promotes photosynthetic gene expression in a pathway that is abolished by the herbicide norflurazon. Far-red light (FR) pretreatment and transfer to white light also results in plastid damage and loss of photosynthetic gene expression. Here, we investigated whether norflurazon and FR pretreatment affect the same retrograde signal.

We used transcriptome analysis and real-time reverse transcription polymerase chain reaction (RT-PCR) to analyse the effects of these treatments on nuclear gene expression in various Arabidopsis (Arabidopsis thaliana) retrograde signalling mutants.

Results showed that the two treatments inhibited largely different nuclear gene sets, suggesting that they affected different retrograde signals. Moreover, FR pretreatment resulted in singlet oxygen (1O2) production and a rapid inhibition of photosynthetic gene expression. This inhibition was partially blocked in the executer1executer2 mutant, which is impaired in 1O2 signalling.

Our data support a new model in which a 1O2 retrograde signal, generated by chlorophyll precursors, inhibits expression of key photosynthetic and chlorophyll synthesis genes to prevent photo-oxidative damage during de-etiolation. Such a signal would provide a counterbalance to the positive haem-related signal to fine tune regulation of chloroplast biogenesis.
0028-646X
1168-1180
Page, Mike T.
c248f4b0-79ae-4ab3-a094-8713e947ec9d
McCormac, Alex C.
41fd08ca-de17-4563-a1f8-d35e1f6c0e20
Smith, Alison G.
30d591f0-8608-47bf-89ee-9278c4bef53d
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Page, Mike T.
c248f4b0-79ae-4ab3-a094-8713e947ec9d
McCormac, Alex C.
41fd08ca-de17-4563-a1f8-d35e1f6c0e20
Smith, Alison G.
30d591f0-8608-47bf-89ee-9278c4bef53d
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b

Page, Mike T., McCormac, Alex C., Smith, Alison G. and Terry, Matthew J. (2017) Singlet oxygen initiates a plastid signal controlling photosynthetic gene expression. New Phytologist, 213 (3), 1168-1180. (doi:10.1111/nph.14223). (PMID:27735068)

Record type: Article

Abstract

Retrograde signals from the plastid regulate photosynthesis-associated nuclear genes and are essential to successful chloroplast biogenesis. One model is that a positive haem-related signal promotes photosynthetic gene expression in a pathway that is abolished by the herbicide norflurazon. Far-red light (FR) pretreatment and transfer to white light also results in plastid damage and loss of photosynthetic gene expression. Here, we investigated whether norflurazon and FR pretreatment affect the same retrograde signal.

We used transcriptome analysis and real-time reverse transcription polymerase chain reaction (RT-PCR) to analyse the effects of these treatments on nuclear gene expression in various Arabidopsis (Arabidopsis thaliana) retrograde signalling mutants.

Results showed that the two treatments inhibited largely different nuclear gene sets, suggesting that they affected different retrograde signals. Moreover, FR pretreatment resulted in singlet oxygen (1O2) production and a rapid inhibition of photosynthetic gene expression. This inhibition was partially blocked in the executer1executer2 mutant, which is impaired in 1O2 signalling.

Our data support a new model in which a 1O2 retrograde signal, generated by chlorophyll precursors, inhibits expression of key photosynthetic and chlorophyll synthesis genes to prevent photo-oxidative damage during de-etiolation. Such a signal would provide a counterbalance to the positive haem-related signal to fine tune regulation of chloroplast biogenesis.

Text
nph14223.pdf - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 19 August 2016
e-pub ahead of print date: 13 October 2016
Published date: February 2017
Organisations: Environmental

Identifiers

Local EPrints ID: 401595
URI: http://eprints.soton.ac.uk/id/eprint/401595
ISSN: 0028-646X
PURE UUID: c5bbb079-d8a3-4511-b535-59458ff8496d
ORCID for Matthew J. Terry: ORCID iD orcid.org/0000-0001-5002-2708

Catalogue record

Date deposited: 14 Oct 2016 15:19
Last modified: 15 Mar 2024 02:52

Export record

Altmetrics

Contributors

Author: Mike T. Page
Author: Alex C. McCormac
Author: Alison G. Smith

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×