The University of Southampton
University of Southampton Institutional Repository

Droplets generation and sampling on demand with peristaltic pumping systems

Droplets generation and sampling on demand with peristaltic pumping systems
Droplets generation and sampling on demand with peristaltic pumping systems
In the last few decades, droplet microfluidics has been developed as a new area of microfluidics, where samples are compartmentalised in another immiscible phase. With these micro-droplets, Taylor dispersion can be minimised between samples and thorough mixing is easy and fast within the droplets themselves. Moreover, the sample consumptions are comparatively low as the samples are limited within nano-litre, pico-litre or even smaller droplets. Due to these attractive features, droplet microfluidics has been widely used as a platform to study various phenomena in chemistry, biology and physics. Droplets are normally generated in a T-junction or flow-focusing with syringe pumps or other pressure sources. An alternative way to generate droplets is to sequentially aspirate aqueous samples and carrier oil under negative pressures. Both of the typical T-junction methods and the current aspiration methods, have limitations in freely introducing/collecting samples into designed droplets, such as continuous sampling where introduction of samples does not affect the droplet generation, and in-situ sampling where samples from environment can be directly introduced into droplets. This ‘sample in’ problem is still a challenge in droplet microfluidics.

This thesis addresses the two droplets sampling issues in droplet microfluidics: continuous droplet sampling, and in-situ droplet sampling. To solve the first issue, a novel microfluidic platform was engineered which includes aspiration droplet generators, a peristaltic pumping system and a feedback system which is used to synchronise droplet generation with pulsations of flowrates from the peristaltic pump. The demonstration of this platform successfully shows the capability of continuously generating and pumping droplets. To solve the second issue, a micro peristaltic pump was engineered to realise a robust droplet generation method and a direct sample introduction from ‘out-world’ to chip. The results show that this device is capable of generating droplets in-situ.
Zhang, Yu
a851ce00-2181-4b85-bb62-50fcbee83a95
Zhang, Yu
a851ce00-2181-4b85-bb62-50fcbee83a95
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa

(2016) Droplets generation and sampling on demand with peristaltic pumping systems. University of Southampton, Faculty of Engineering and the Environment, Doctoral Thesis, 212pp.

Record type: Thesis (Doctoral)

Abstract

In the last few decades, droplet microfluidics has been developed as a new area of microfluidics, where samples are compartmentalised in another immiscible phase. With these micro-droplets, Taylor dispersion can be minimised between samples and thorough mixing is easy and fast within the droplets themselves. Moreover, the sample consumptions are comparatively low as the samples are limited within nano-litre, pico-litre or even smaller droplets. Due to these attractive features, droplet microfluidics has been widely used as a platform to study various phenomena in chemistry, biology and physics. Droplets are normally generated in a T-junction or flow-focusing with syringe pumps or other pressure sources. An alternative way to generate droplets is to sequentially aspirate aqueous samples and carrier oil under negative pressures. Both of the typical T-junction methods and the current aspiration methods, have limitations in freely introducing/collecting samples into designed droplets, such as continuous sampling where introduction of samples does not affect the droplet generation, and in-situ sampling where samples from environment can be directly introduced into droplets. This ‘sample in’ problem is still a challenge in droplet microfluidics.

This thesis addresses the two droplets sampling issues in droplet microfluidics: continuous droplet sampling, and in-situ droplet sampling. To solve the first issue, a novel microfluidic platform was engineered which includes aspiration droplet generators, a peristaltic pumping system and a feedback system which is used to synchronise droplet generation with pulsations of flowrates from the peristaltic pump. The demonstration of this platform successfully shows the capability of continuously generating and pumping droplets. To solve the second issue, a micro peristaltic pump was engineered to realise a robust droplet generation method and a direct sample introduction from ‘out-world’ to chip. The results show that this device is capable of generating droplets in-situ.

PDF
Zhang 23980087 Final E-Thesis for E-Prints.pdf - Other
Available under License University of Southampton Thesis Licence.
Download (6MB)

More information

Published date: 1 March 2016
Organisations: University of Southampton, Mechatronics

Identifiers

Local EPrints ID: 401883
URI: http://eprints.soton.ac.uk/id/eprint/401883
PURE UUID: e68c5956-a2ba-4e13-9845-ee32d6a41bdb

Catalogue record

Date deposited: 01 Dec 2016 14:59
Last modified: 21 Apr 2018 04:01

Export record

Contributors

Author: Yu Zhang
Thesis advisor: Xize Niu

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×