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Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles

Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles
Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles
The transfer of material from phospholipid-coated microbubbles to cell membranes has been hypothesized to play a role in ultrasound-mediated drug delivery. In this study, we employed quantitative fluorescence microscopy techniques to investigate this phenomenon in both artificial and biological membrane bilayers in an acoustofluidic system. The results of the present study provide strong evidence for the transfer of material from microbubble coatings into cell membranes. Our results indicate that transfer of phospholipids alters the organization of molecules in cell membranes, specifically the lipid ordering or packing, which is known to be a key determinant of membrane mechanical properties, protein dynamics, and permeability. We further show that polyethylene-glycol, used in many clinical microbubble formulations, also has a major impact on both membrane lipid ordering and the extent of lipid transfer, and that this occurs even in the absence of ultrasound exposure.
microbubbles, ultrasound, lipid order, membrane bilayers
0142-9612
105-117
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Aron, Miles
4d9c7843-bbe5-4a5d-975f-ea58a09fc621
Sezgin, Erdinc
7b0b5507-2421-46f8-8ef0-04a7bc510348
Bernardino de la Serna, Jorge
d568eb8d-3a54-4fa0-bbf0-7429300aa31f
Kuimova, Marina
19d0463b-c396-4717-8556-47dd38d4de49
Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Aron, Miles
4d9c7843-bbe5-4a5d-975f-ea58a09fc621
Sezgin, Erdinc
7b0b5507-2421-46f8-8ef0-04a7bc510348
Bernardino de la Serna, Jorge
d568eb8d-3a54-4fa0-bbf0-7429300aa31f
Kuimova, Marina
19d0463b-c396-4717-8556-47dd38d4de49
Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

Carugo, Dario, Aron, Miles, Sezgin, Erdinc, Bernardino de la Serna, Jorge, Kuimova, Marina, Eggeling, Christian and Stride, Eleanor (2017) Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles. Biomaterials, 113, 105-117. (doi:10.1016/j.biomaterials.2016.10.034).

Record type: Article

Abstract

The transfer of material from phospholipid-coated microbubbles to cell membranes has been hypothesized to play a role in ultrasound-mediated drug delivery. In this study, we employed quantitative fluorescence microscopy techniques to investigate this phenomenon in both artificial and biological membrane bilayers in an acoustofluidic system. The results of the present study provide strong evidence for the transfer of material from microbubble coatings into cell membranes. Our results indicate that transfer of phospholipids alters the organization of molecules in cell membranes, specifically the lipid ordering or packing, which is known to be a key determinant of membrane mechanical properties, protein dynamics, and permeability. We further show that polyethylene-glycol, used in many clinical microbubble formulations, also has a major impact on both membrane lipid ordering and the extent of lipid transfer, and that this occurs even in the absence of ultrasound exposure.

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More information

Accepted/In Press date: 23 October 2016
e-pub ahead of print date: 24 October 2016
Published date: January 2017
Keywords: microbubbles, ultrasound, lipid order, membrane bilayers
Organisations: Bioengineering Group, Mechatronics

Identifiers

Local EPrints ID: 401933
URI: https://eprints.soton.ac.uk/id/eprint/401933
ISSN: 0142-9612
PURE UUID: c6204573-b983-40c7-9e40-cda38eae549a

Catalogue record

Date deposited: 25 Oct 2016 12:59
Last modified: 02 May 2018 04:01

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