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Omega-3 fatty acids do not alter P-wave parameters in electrocardiogram or expression of atrial connexins in patients undergoing coronary artery bypass surgery.

Omega-3 fatty acids do not alter P-wave parameters in electrocardiogram or expression of atrial connexins in patients undergoing coronary artery bypass surgery.
Omega-3 fatty acids do not alter P-wave parameters in electrocardiogram or expression of atrial connexins in patients undergoing coronary artery bypass surgery.
Aims: We previously reported omega-3 polyunsaturated fatty acids (n-3PUFAs) supplementation does not reduce atrial fibrillation (AF) following coronary artery bypass graft (CABG) surgery. The aim of the present study is to evaluate the impact of n-3 PUFAs on electrocardiogram (ECG) atrial arrhythmic markers and compare with expression of gap-junction proteins, Connexins.

Methods and results: Subset of clinical trial subjects with right atrial sampling during CABG surgery included. Twelve-lead ECG performed at recruitment and at surgery [after supplementation with n-3 PUFA (?1.8 g/day) or matched placebo] for ?14 days. Electrocardiograms analysed for maximum P-wave duration (P-max) and difference between P-max and minimum P-wave duration, P-wave dispersion (PWD). Right atrial specimens analysed for expression of Connexins 40 and 43 using real-time quantitative polymerase chain reaction (qPCR) and western blot. Serum levels of n-3 PUFA at baseline, at surgery, and atrial tissue levels at surgery collated from file. Postoperative AF was quantified by analysing data from stored continuous electrograms. A total of 61 patients (n-3 PUFA 34, Placebo 27) had ECG analysis and AF burden, of which 52 patients (26 in each group) had qPCR and 16 (8 in each group) had western blot analyses for Connexins 40 and 43. No difference between the two groups in ECG parameters or expression of Connexin 40 or 43. P-wave dispersion in the preoperative ECG independently predicted occurrence of AF following CABG surgery.

Conclusions: Omega-3 polyunsaturated fatty acids supplementation does not alter pro-arrhythmic P-wave parameters in ECG or connexin expression in human atrium with no effect on the incidence of AF following CABG surgery.
1099-5129
1521-1527
Saravanan, P.
617bf087-f052-46a2-88ce-bc016055d701
West, A.L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Bridgewater, B.
ba260acb-9624-46ad-8b6e-5e3ca9fa21e2
Davidson, N.C.
b5461d0f-890a-4f5d-81ca-8551abb72587
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Dobrzynsky, H.
31418de7-df36-4b86-82e5-e2350fff7d09
Trafford, A.
1874e6e6-bbf0-48f6-b996-b0e9d0575b0c
O'Neill, S.C.
fca4381e-a177-4887-be52-2a569b7db51a
Saravanan, P.
617bf087-f052-46a2-88ce-bc016055d701
West, A.L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Bridgewater, B.
ba260acb-9624-46ad-8b6e-5e3ca9fa21e2
Davidson, N.C.
b5461d0f-890a-4f5d-81ca-8551abb72587
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Dobrzynsky, H.
31418de7-df36-4b86-82e5-e2350fff7d09
Trafford, A.
1874e6e6-bbf0-48f6-b996-b0e9d0575b0c
O'Neill, S.C.
fca4381e-a177-4887-be52-2a569b7db51a

Saravanan, P., West, A.L., Bridgewater, B., Davidson, N.C., Calder, P.C., Dobrzynsky, H., Trafford, A. and O'Neill, S.C. (2016) Omega-3 fatty acids do not alter P-wave parameters in electrocardiogram or expression of atrial connexins in patients undergoing coronary artery bypass surgery. Europace, 18 (10), 1521-1527. (doi:10.1093/europace/euv398). (PMID:26850746)

Record type: Article

Abstract

Aims: We previously reported omega-3 polyunsaturated fatty acids (n-3PUFAs) supplementation does not reduce atrial fibrillation (AF) following coronary artery bypass graft (CABG) surgery. The aim of the present study is to evaluate the impact of n-3 PUFAs on electrocardiogram (ECG) atrial arrhythmic markers and compare with expression of gap-junction proteins, Connexins.

Methods and results: Subset of clinical trial subjects with right atrial sampling during CABG surgery included. Twelve-lead ECG performed at recruitment and at surgery [after supplementation with n-3 PUFA (?1.8 g/day) or matched placebo] for ?14 days. Electrocardiograms analysed for maximum P-wave duration (P-max) and difference between P-max and minimum P-wave duration, P-wave dispersion (PWD). Right atrial specimens analysed for expression of Connexins 40 and 43 using real-time quantitative polymerase chain reaction (qPCR) and western blot. Serum levels of n-3 PUFA at baseline, at surgery, and atrial tissue levels at surgery collated from file. Postoperative AF was quantified by analysing data from stored continuous electrograms. A total of 61 patients (n-3 PUFA 34, Placebo 27) had ECG analysis and AF burden, of which 52 patients (26 in each group) had qPCR and 16 (8 in each group) had western blot analyses for Connexins 40 and 43. No difference between the two groups in ECG parameters or expression of Connexin 40 or 43. P-wave dispersion in the preoperative ECG independently predicted occurrence of AF following CABG surgery.

Conclusions: Omega-3 polyunsaturated fatty acids supplementation does not alter pro-arrhythmic P-wave parameters in ECG or connexin expression in human atrium with no effect on the incidence of AF following CABG surgery.

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More information

Accepted/In Press date: 5 October 2015
e-pub ahead of print date: 5 February 2016
Published date: October 2016
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 402376
URI: http://eprints.soton.ac.uk/id/eprint/402376
ISSN: 1099-5129
PURE UUID: 5eb66fb5-f23e-469d-825e-9343bc9567a7
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 07 Nov 2016 16:23
Last modified: 11 Mar 2021 02:35

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