Rare genetic variation in UNC13A may modify survival in amyotrophic lateral sclerosis
Rare genetic variation in UNC13A may modify survival in amyotrophic lateral sclerosis
Our objective was to identify whether rare genetic variation in amyotrophic lateral sclerosis (ALS) candidate survival genes modifies ALS survival. Candidate genes were selected based on evidence for modifying ALS survival. Each tail of the extreme 1.5% of survival was selected from the UK MND DNA Bank and all samples available underwent whole genome sequencing. A replication set from the Netherlands was used for validation. Sequences of candidate survival genes were extracted and variants passing quality control with a minor allele frequency ?0.05 were selected for association testing. Analysis was by burden testing using SKAT.
Candidate survival genes UNC13A, KIFAP3, and EPHA4 were tested for association in a UK sample comprising 25 short survivors and 25 long survivors. Results showed that only SNVs in UNC13A were associated with survival (p = 6.57?×?10?3). SNV rs10419420:G?>?A was found exclusively in long survivors (3/25) and rs4808092:G?>?A exclusively in short survivors (4/25). These findings were not replicated in a Dutch sample. In conclusion, population specific rare variants of UNC13A may modulate survival in ALS.
1-7
Gaastra, Benjamin
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Shatunov, Aleksey
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Pulit, Sara
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Jones, Ashley R.
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Sproviero, William
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Gillett, Alexandra
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Chen, Zhongbo
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Kirby, Janine
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Fogh, Isabella
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Powell, John F.
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Leigh, P. Nigel
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Morrison, Karen E.
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Shaw, Pamela J.
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Shaw, Christopher E.
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van den Berg, Leonard H.
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Veldink, Jan H.
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Lewis, Cathryn M.
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Al-Chalabi, Ammar
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Gaastra, Benjamin
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Shatunov, Aleksey
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Pulit, Sara
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Jones, Ashley R.
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Sproviero, William
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Gillett, Alexandra
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Chen, Zhongbo
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Kirby, Janine
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Fogh, Isabella
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Powell, John F.
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Leigh, P. Nigel
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Morrison, Karen E.
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Shaw, Pamela J.
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Shaw, Christopher E.
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van den Berg, Leonard H.
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Veldink, Jan H.
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Lewis, Cathryn M.
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Al-Chalabi, Ammar
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Gaastra, Benjamin, Shatunov, Aleksey, Pulit, Sara, Jones, Ashley R., Sproviero, William, Gillett, Alexandra, Chen, Zhongbo, Kirby, Janine, Fogh, Isabella, Powell, John F., Leigh, P. Nigel, Morrison, Karen E., Shaw, Pamela J., Shaw, Christopher E., van den Berg, Leonard H., Veldink, Jan H., Lewis, Cathryn M. and Al-Chalabi, Ammar
(2016)
Rare genetic variation in UNC13A may modify survival in amyotrophic lateral sclerosis.
Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, .
(doi:10.1080/21678421.2016.1213852).
Abstract
Our objective was to identify whether rare genetic variation in amyotrophic lateral sclerosis (ALS) candidate survival genes modifies ALS survival. Candidate genes were selected based on evidence for modifying ALS survival. Each tail of the extreme 1.5% of survival was selected from the UK MND DNA Bank and all samples available underwent whole genome sequencing. A replication set from the Netherlands was used for validation. Sequences of candidate survival genes were extracted and variants passing quality control with a minor allele frequency ?0.05 were selected for association testing. Analysis was by burden testing using SKAT.
Candidate survival genes UNC13A, KIFAP3, and EPHA4 were tested for association in a UK sample comprising 25 short survivors and 25 long survivors. Results showed that only SNVs in UNC13A were associated with survival (p = 6.57?×?10?3). SNV rs10419420:G?>?A was found exclusively in long survivors (3/25) and rs4808092:G?>?A exclusively in short survivors (4/25). These findings were not replicated in a Dutch sample. In conclusion, population specific rare variants of UNC13A may modulate survival in ALS.
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Accepted/In Press date: 13 June 2016
e-pub ahead of print date: 1 September 2016
Organisations:
Faculty of Medicine
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Local EPrints ID: 402879
URI: http://eprints.soton.ac.uk/id/eprint/402879
ISSN: 2167-8421
PURE UUID: 85d239b5-a733-4839-8b78-c5cfed7b3a13
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Date deposited: 15 Nov 2016 09:17
Last modified: 11 Feb 2022 02:55
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Contributors
Author:
Aleksey Shatunov
Author:
Sara Pulit
Author:
Ashley R. Jones
Author:
William Sproviero
Author:
Alexandra Gillett
Author:
Zhongbo Chen
Author:
Janine Kirby
Author:
Isabella Fogh
Author:
John F. Powell
Author:
P. Nigel Leigh
Author:
Karen E. Morrison
Author:
Pamela J. Shaw
Author:
Christopher E. Shaw
Author:
Leonard H. van den Berg
Author:
Jan H. Veldink
Author:
Cathryn M. Lewis
Author:
Ammar Al-Chalabi
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