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Transmembrane fluoride transport: direct measurement and selectivity studies

Transmembrane fluoride transport: direct measurement and selectivity studies
Transmembrane fluoride transport: direct measurement and selectivity studies
Fluoride has been overlooked as a target in the development of synthetic anion transporters despite natural fluoride transport channels being recently discovered. In this paper we report the direct measurement of fluoride bilayer transport facilitated by a series of strapped calix[4]pyrroles and show that these compounds facilitate transport via an electrogenic mechanism (determined using valinomycin and monensin coupled transport assays and an additional osmotic response assay). An HPTS transport assay was used to quantify this electrogenic process and assess the interference of naturally occurring fatty acids with the transport process and Cl- over H+/OH- transport selectivity.
0002-7863
16515-16522
Clarke, Harriet
f5c240ce-625a-47b4-a835-8b7cb44681d2
Howe, Ethan
be05ae9a-02e0-443b-97b2-d65cc387af40
Wu, Xin
65360fe8-d25a-4652-b7e4-40d513b57e7e
Sommer, Fabian
45aecf6d-63ff-4530-8deb-27d8a5b36604
Yano, Masafumi
feec7c9e-6bff-47fd-88d6-3ac47deeb8e6
Light, Mark
cf57314e-6856-491b-a8d2-2dffc452e161
Kubik, Stefan
a6966f22-2ddf-409a-afc9-952299a83a48
Gale, Philip
c840b7e9-6847-4843-91af-fa0f8563d943
Clarke, Harriet
f5c240ce-625a-47b4-a835-8b7cb44681d2
Howe, Ethan
be05ae9a-02e0-443b-97b2-d65cc387af40
Wu, Xin
65360fe8-d25a-4652-b7e4-40d513b57e7e
Sommer, Fabian
45aecf6d-63ff-4530-8deb-27d8a5b36604
Yano, Masafumi
feec7c9e-6bff-47fd-88d6-3ac47deeb8e6
Light, Mark
cf57314e-6856-491b-a8d2-2dffc452e161
Kubik, Stefan
a6966f22-2ddf-409a-afc9-952299a83a48
Gale, Philip
c840b7e9-6847-4843-91af-fa0f8563d943

Clarke, Harriet, Howe, Ethan, Wu, Xin, Sommer, Fabian, Yano, Masafumi, Light, Mark, Kubik, Stefan and Gale, Philip (2016) Transmembrane fluoride transport: direct measurement and selectivity studies. Journal of the American Chemical Society, 138, 16515-16522. (doi:10.1021/jacs.6b10694).

Record type: Article

Abstract

Fluoride has been overlooked as a target in the development of synthetic anion transporters despite natural fluoride transport channels being recently discovered. In this paper we report the direct measurement of fluoride bilayer transport facilitated by a series of strapped calix[4]pyrroles and show that these compounds facilitate transport via an electrogenic mechanism (determined using valinomycin and monensin coupled transport assays and an additional osmotic response assay). An HPTS transport assay was used to quantify this electrogenic process and assess the interference of naturally occurring fatty acids with the transport process and Cl- over H+/OH- transport selectivity.

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More information

Accepted/In Press date: 22 November 2016
e-pub ahead of print date: 7 December 2016
Published date: 7 December 2016
Organisations: Organic Chemistry: SCF

Identifiers

Local EPrints ID: 403218
URI: http://eprints.soton.ac.uk/id/eprint/403218
ISSN: 0002-7863
PURE UUID: fadc17cd-55e8-433a-bfb0-84c5d6f33e62
ORCID for Mark Light: ORCID iD orcid.org/0000-0002-0585-0843
ORCID for Philip Gale: ORCID iD orcid.org/0000-0001-9751-4910

Catalogue record

Date deposited: 28 Nov 2016 14:55
Last modified: 16 Mar 2024 03:16

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Contributors

Author: Harriet Clarke
Author: Ethan Howe
Author: Xin Wu
Author: Fabian Sommer
Author: Masafumi Yano
Author: Mark Light ORCID iD
Author: Stefan Kubik
Author: Philip Gale ORCID iD

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