The University of Southampton
University of Southampton Institutional Repository

Microfluidic system for chemiluminescence characterisation

Mosayebi, Ali (2013) Microfluidic system for chemiluminescence characterisation University of Southampton, School of Electronics and Computer Science: Optoelectronics Research Centre, Masters Thesis .

Record type: Thesis (Masters)

Abstract

Microfluidic technology (generally lab-on-a-chip) has been focused area of research since 90s and the main goal of this technology is to develop different chemical and biological techniques. It is quicker than the correspondent traditional techniques and consumes small volumes of material.
One of the important functions in micro scale fluidic channels is mixing that its characteristics affected by scale because the viscous forces are more than inertial forces in this range of dimensions that provides laminar flow inside the channel; in this scale the mixing is defined by diffusion rather than turbulence.
This dissertation presents the design of a microfluidic device and employing a combination of rapid prototype technology with polydimethylsiloxane (PDMS) for the fabrication. After the fabrication, chemiluminescence phenomenon is tested inside the device and the resultant images from the charge-coupled-device (CCD) camera are analysed using numerical analysis and image processing.
Two sets of tests are performed for characterising the chemiluminescence in fabricated device. Preliminary tests to check the device and the chemicals’ behaviour and the actual test. In order to find the limit of detection, which is obtained as lower than 4 x 10-6 g of the dominant chemical (luminol) concentration in 100 ml of the solution, depends upon different parameters such as flow rate and the concentration of luminol are checked. The effect of the flow rate and the concentration on the maximum value and the decay length of the light intensity along the fluidic missing channels are then discussed.
In the final part of the project, nanoparticles are used to improve the intensity of the light in the channel in order to improve the limit of detection of the system.

Text am10g11 MSc Dissertation.pdf - Other
Download (2MB)

More information

Published date: May 2013
Organisations: University of Southampton, Optoelectronics Research Centre

Identifiers

Local EPrints ID: 403346
URI: http://eprints.soton.ac.uk/id/eprint/403346
PURE UUID: 58105b71-58c7-4a91-aebf-ade80d59f11c
ORCID for James Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 28 Nov 2016 09:15
Last modified: 17 Oct 2017 16:31

Export record

Contributors

Author: Ali Mosayebi
Thesis advisor: James Wilkinson ORCID iD
Thesis advisor: Jonathan Butement
Thesis advisor: ALINA KARABCHEVSKY

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×