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Structure and function of methanogenic microbial communities in soils from flooded rice and upland soybean fields from Sanjiang plain, NE China

Structure and function of methanogenic microbial communities in soils from flooded rice and upland soybean fields from Sanjiang plain, NE China
Structure and function of methanogenic microbial communities in soils from flooded rice and upland soybean fields from Sanjiang plain, NE China
About 50 years ago, most of the natural wetlands in northeast China, the Sanjiang plain, were converted to either flooded rice fields or to upland soybean fields. After the conversion, natural wetland soils were either managed as artificial wetland or as drained upland resulting in soil microbial community changes. The purpose of our study was to understand how methanogenic microbial communities and their functions had changed in the two different soils upon conversion, and whether these communities now exhibit different resistance/resilience to drying and rewetting. Therefore, we determined function, abundance and composition of the methanogenic archaeal and bacterial communities in two soils reclaimed from a Carex wetland 25 years ago. We incubated the soils under anoxic conditions and measured the rates and pathways of CH4 production by analyzing concentration and ?13C of CH4 and acetate in the presence and absence of methyl fluoride, an inhibitor of aceticlastic methanogenesis. We also analyzed the abundance of bacterial and archaeal 16S rRNA genes, and of mcrA (coding for a subunit of the methyl coenzyme M reductase) using qPCR. The composition of the archaeal and bacterial 16S rRNA genes was determined by using MiSeq illumina sequencing. Our results showed clear differences in structure and function of methanogenic archaeal communities in rice field soil versus upland soil. Furthermore, in both soils composition of bacteria and archaea changed after artificial drying and became less diverse. The archaeal and bacterial signature species in the two soils were also different. However, functional changes were similar, with rates of CH4 production and contribution of aceticlastic methanogenesis decreasing upon drying and rewetting in both soils.
0038-0717
81-91
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96
Klose, Melanie
40360195-8086-4396-a2fd-be227acbe099
Ma, Ke
7c09ef9b-873b-472f-9424-7e99a4875f8f
Lu, Yahai
817deacf-4d36-4226-92ca-3c321d7a28ab
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96
Klose, Melanie
40360195-8086-4396-a2fd-be227acbe099
Ma, Ke
7c09ef9b-873b-472f-9424-7e99a4875f8f
Lu, Yahai
817deacf-4d36-4226-92ca-3c321d7a28ab

Hernandez Garcia, Marcela, Conrad, Ralf, Klose, Melanie, Ma, Ke and Lu, Yahai (2017) Structure and function of methanogenic microbial communities in soils from flooded rice and upland soybean fields from Sanjiang plain, NE China. Soil Biology and Biochemistry, 105, 81-91. (doi:10.1016/j.soilbio.2016.11.010).

Record type: Article

Abstract

About 50 years ago, most of the natural wetlands in northeast China, the Sanjiang plain, were converted to either flooded rice fields or to upland soybean fields. After the conversion, natural wetland soils were either managed as artificial wetland or as drained upland resulting in soil microbial community changes. The purpose of our study was to understand how methanogenic microbial communities and their functions had changed in the two different soils upon conversion, and whether these communities now exhibit different resistance/resilience to drying and rewetting. Therefore, we determined function, abundance and composition of the methanogenic archaeal and bacterial communities in two soils reclaimed from a Carex wetland 25 years ago. We incubated the soils under anoxic conditions and measured the rates and pathways of CH4 production by analyzing concentration and ?13C of CH4 and acetate in the presence and absence of methyl fluoride, an inhibitor of aceticlastic methanogenesis. We also analyzed the abundance of bacterial and archaeal 16S rRNA genes, and of mcrA (coding for a subunit of the methyl coenzyme M reductase) using qPCR. The composition of the archaeal and bacterial 16S rRNA genes was determined by using MiSeq illumina sequencing. Our results showed clear differences in structure and function of methanogenic archaeal communities in rice field soil versus upland soil. Furthermore, in both soils composition of bacteria and archaea changed after artificial drying and became less diverse. The archaeal and bacterial signature species in the two soils were also different. However, functional changes were similar, with rates of CH4 production and contribution of aceticlastic methanogenesis decreasing upon drying and rewetting in both soils.

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Accepted/In Press date: 9 November 2016
e-pub ahead of print date: 21 November 2016
Published date: February 2017
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 403907
URI: http://eprints.soton.ac.uk/id/eprint/403907
ISSN: 0038-0717
PURE UUID: c5b3a99c-2b96-4062-9390-83a21af54289

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Date deposited: 16 Dec 2016 10:11
Last modified: 15 Mar 2024 06:09

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Contributors

Author: Marcela Hernandez Garcia
Author: Ralf Conrad
Author: Melanie Klose
Author: Ke Ma
Author: Yahai Lu

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