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In vivo and in vitro studies of the role of lyophilised blond Lager beer and some bioactive components in the modulation of degenerative processes

In vivo and in vitro studies of the role of lyophilised blond Lager beer and some bioactive components in the modulation of degenerative processes
In vivo and in vitro studies of the role of lyophilised blond Lager beer and some bioactive components in the modulation of degenerative processes
The aim of the present study was to examine the nutraceutic potential of lyophilised blond Lager beer, as well as two of its bioactive components: xanthohumol and folic acid. Several toxicity, antitoxicity, genotoxicity, antigenotoxicity and longevity endpoints were checked in the SMART in vivo Drosophila system. Cytotoxicity in HL-60 promyelocytic and NIH3T3 mouse fibroblasts cells, proapototic DNA fragmentation, comet assay, macroautophagy activity and methylation status were tested in in vitro assays. Lyophilised blond Lager beer could be proposed as a substance with an important nutraceutical value, because of its hopeful results as a lifespan promoter, DNA protection against free radicals in the animal model and its chemopreventive activity in HL-60 cells and enhacer of macroautophagy at moderate doses in NIH3T3 inmortal cells. Only xanthohumol mimics the biological activities found in lyophilised beer.
1756-4646
274-294
Merinas-Amo, Tania
90712d2f-8e89-400e-9e3b-3e158860e4bb
Tasset-Cuevas, Inmaculada
2d29576a-278c-4451-81cd-4cc33ca3149e
Díaz-Carretero, Antonio M.
762031be-802e-44ad-836d-d092fd41851a
Alonso-Moraga, Ángeles
9bbc2fa5-c85a-4646-89af-460e78d33000
Calahorro, Fernando
dddfa373-d3cc-433f-8851-9ca37f2f3950
Merinas-Amo, Tania
90712d2f-8e89-400e-9e3b-3e158860e4bb
Tasset-Cuevas, Inmaculada
2d29576a-278c-4451-81cd-4cc33ca3149e
Díaz-Carretero, Antonio M.
762031be-802e-44ad-836d-d092fd41851a
Alonso-Moraga, Ángeles
9bbc2fa5-c85a-4646-89af-460e78d33000
Calahorro, Fernando
dddfa373-d3cc-433f-8851-9ca37f2f3950

Merinas-Amo, Tania, Tasset-Cuevas, Inmaculada, Díaz-Carretero, Antonio M., Alonso-Moraga, Ángeles and Calahorro, Fernando (2016) In vivo and in vitro studies of the role of lyophilised blond Lager beer and some bioactive components in the modulation of degenerative processes. Journal of Functional Foods, 27, 274-294. (doi:10.1016/j.jff.2016.09.014).

Record type: Article

Abstract

The aim of the present study was to examine the nutraceutic potential of lyophilised blond Lager beer, as well as two of its bioactive components: xanthohumol and folic acid. Several toxicity, antitoxicity, genotoxicity, antigenotoxicity and longevity endpoints were checked in the SMART in vivo Drosophila system. Cytotoxicity in HL-60 promyelocytic and NIH3T3 mouse fibroblasts cells, proapototic DNA fragmentation, comet assay, macroautophagy activity and methylation status were tested in in vitro assays. Lyophilised blond Lager beer could be proposed as a substance with an important nutraceutical value, because of its hopeful results as a lifespan promoter, DNA protection against free radicals in the animal model and its chemopreventive activity in HL-60 cells and enhacer of macroautophagy at moderate doses in NIH3T3 inmortal cells. Only xanthohumol mimics the biological activities found in lyophilised beer.

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Accepted/In Press date: 16 September 2016
e-pub ahead of print date: 29 September 2016
Published date: December 2016
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 403970
URI: http://eprints.soton.ac.uk/id/eprint/403970
ISSN: 1756-4646
PURE UUID: dd8865de-8db0-4671-9230-e3495632066f
ORCID for Fernando Calahorro: ORCID iD orcid.org/0000-0003-0659-7728

Catalogue record

Date deposited: 19 Dec 2016 10:04
Last modified: 10 Dec 2019 01:35

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