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Low-cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay

Low-cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay
Low-cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay
We describe a low cost thin-film transistor (TFT) nanoribbon sensor for detection of the inflammatory biomarker C-reactive protein (CRP) in human serum via a miniature bead-based enzyme-linked immunosorbent assay (ELISA). The TFT nanoribbon sensor measures the reaction products from the ELISA via pH changes. The bead-based ELISA decouples the protein functionalization steps from the sensor surface, increasing the signal and simplifying the assay. The ability to directly sense proteins in human serum in this way overcomes the Debye length limitation associated with nanowire and nanoribbon biosensors. Compared to classically fabricated nanowires, the TFT nanoribbon sensors are simple, extremely easy to fabricate, and should therefore be much cheaper to manufacture. TFT nanoribbon sensors, configured to measure pH, were used for quantitative detection of CRP spiked into human serum at concentrations as low as 0.2 ng/mL, which is 10,000 times lower than needed for diagnostic purposes, providing the potential for applications that require very high sensitivity.
0003-2700
4872-4878
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Zeimpekis, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Sun Kai
5c758916-a01b-435d-ab57-24eb7e5701d5
Anderson, Sally
bb9101ff-f8f1-432a-9dfb-8dc46e52b006
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Zeimpekis, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Sun Kai
5c758916-a01b-435d-ab57-24eb7e5701d5
Anderson, Sally
bb9101ff-f8f1-432a-9dfb-8dc46e52b006
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Hu, Chunxiao, Zeimpekis, Ioannis, Sun, Sun Kai, Anderson, Sally, Ashburn, Peter and Morgan, Hywel (2016) Low-cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay. Analytical Chemistry, 88 (9), 4872-4878. (doi:10.1021/acs.analchem.6b00702).

Record type: Article

Abstract

We describe a low cost thin-film transistor (TFT) nanoribbon sensor for detection of the inflammatory biomarker C-reactive protein (CRP) in human serum via a miniature bead-based enzyme-linked immunosorbent assay (ELISA). The TFT nanoribbon sensor measures the reaction products from the ELISA via pH changes. The bead-based ELISA decouples the protein functionalization steps from the sensor surface, increasing the signal and simplifying the assay. The ability to directly sense proteins in human serum in this way overcomes the Debye length limitation associated with nanowire and nanoribbon biosensors. Compared to classically fabricated nanowires, the TFT nanoribbon sensors are simple, extremely easy to fabricate, and should therefore be much cheaper to manufacture. TFT nanoribbon sensors, configured to measure pH, were used for quantitative detection of CRP spiked into human serum at concentrations as low as 0.2 ng/mL, which is 10,000 times lower than needed for diagnostic purposes, providing the potential for applications that require very high sensitivity.

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Accepted/In Press date: 1 April 2016
e-pub ahead of print date: 1 April 2016
Published date: 3 May 2016
Organisations: Optoelectronics Research Centre, Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 404105
URI: https://eprints.soton.ac.uk/id/eprint/404105
ISSN: 0003-2700
PURE UUID: b8264298-4aa9-47f0-990d-03cb311e35c4
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 22 Dec 2016 09:55
Last modified: 17 Sep 2019 05:08

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