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Spatial and temporal specificity of Ca2+ signalling in Chlamydomonas reinhardtii in response to osmotic stress

Spatial and temporal specificity of Ca2+ signalling in Chlamydomonas reinhardtii in response to osmotic stress
Spatial and temporal specificity of Ca2+ signalling in Chlamydomonas reinhardtii in response to osmotic stress
Ca2+?dependent signalling processes enable plants to perceive and respond to diverse environmental stressors, such as osmotic stress. A clear understanding of the role of spatiotemporal Ca2+ signalling in green algal lineages is necessary in order to understand how the Ca2+ signalling machinery has evolved in land plants.

We used single?cell imaging of Ca2+?responsive fluorescent dyes in the unicellular green alga Chlamydomonas reinhardtii to examine the specificity of spatial and temporal dynamics of Ca2+ elevations in the cytosol and flagella in response to salinity and osmotic stress.

We found that salt stress induced a single Ca2+ elevation that was modulated by the strength of the stimulus and originated in the apex of the cell, spreading as a fast Ca2+ wave. By contrast, hypo?osmotic stress induced a series of repetitive Ca2+ elevations in the cytosol that were spatially uniform. Hypo?osmotic stimuli also induced Ca2+ elevations in the flagella that occurred independently from those in the cytosol.

Our results indicate that the requirement for Ca2+ signalling in response to osmotic stress is conserved between land plants and green algae, but the distinct spatial and temporal dynamics of osmotic Ca2+ elevations in C. reinhardtii suggest important mechanistic differences between the two lineages.
calcium, chlamydomonas reinhardtii, flagella, green algae, osmotic stress, signalling
0028-646X
920-933
Bickerton, Peter
1e7e35e2-81b8-4ff4-b2b1-e238f492a605
Sello, Simone
3167333c-6bba-4536-af6c-7576f5470857
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Pittman, Jon K.
186ccb17-c717-43d5-b583-293c7cc986ea
Wheeler, Glen L.
80ee477b-ceb3-4051-923c-399098bb746a
Bickerton, Peter
1e7e35e2-81b8-4ff4-b2b1-e238f492a605
Sello, Simone
3167333c-6bba-4536-af6c-7576f5470857
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Pittman, Jon K.
186ccb17-c717-43d5-b583-293c7cc986ea
Wheeler, Glen L.
80ee477b-ceb3-4051-923c-399098bb746a

Bickerton, Peter, Sello, Simone, Brownlee, Colin, Pittman, Jon K. and Wheeler, Glen L. (2016) Spatial and temporal specificity of Ca2+ signalling in Chlamydomonas reinhardtii in response to osmotic stress. New Phytologist, 212 (4), 920-933. (doi:10.1111/nph.14128). (PMID:27516045)

Record type: Article

Abstract

Ca2+?dependent signalling processes enable plants to perceive and respond to diverse environmental stressors, such as osmotic stress. A clear understanding of the role of spatiotemporal Ca2+ signalling in green algal lineages is necessary in order to understand how the Ca2+ signalling machinery has evolved in land plants.

We used single?cell imaging of Ca2+?responsive fluorescent dyes in the unicellular green alga Chlamydomonas reinhardtii to examine the specificity of spatial and temporal dynamics of Ca2+ elevations in the cytosol and flagella in response to salinity and osmotic stress.

We found that salt stress induced a single Ca2+ elevation that was modulated by the strength of the stimulus and originated in the apex of the cell, spreading as a fast Ca2+ wave. By contrast, hypo?osmotic stress induced a series of repetitive Ca2+ elevations in the cytosol that were spatially uniform. Hypo?osmotic stimuli also induced Ca2+ elevations in the flagella that occurred independently from those in the cytosol.

Our results indicate that the requirement for Ca2+ signalling in response to osmotic stress is conserved between land plants and green algae, but the distinct spatial and temporal dynamics of osmotic Ca2+ elevations in C. reinhardtii suggest important mechanistic differences between the two lineages.

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Accepted/In Press date: 25 June 2016
e-pub ahead of print date: 12 August 2016
Published date: December 2016
Keywords: calcium, chlamydomonas reinhardtii, flagella, green algae, osmotic stress, signalling
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 404833
URI: https://eprints.soton.ac.uk/id/eprint/404833
ISSN: 0028-646X
PURE UUID: acabd192-2984-44b4-b4e2-1ec23b8586f5

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Date deposited: 17 Jan 2017 10:08
Last modified: 14 Aug 2019 17:56

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Contributors

Author: Peter Bickerton
Author: Simone Sello
Author: Colin Brownlee
Author: Jon K. Pittman
Author: Glen L. Wheeler

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