Variable leucine-rich repeats of tomato disease resistance genes Cf-2
and Cf-5 determine specificity
Variable leucine-rich repeats of tomato disease resistance genes Cf-2
and Cf-5 determine specificity
The tomato Cf-2 and Cf-5 genes confer race specific resistance to infection by the leaf mould pathogen Cladosporium fulvum. The encoded proteins induce a defence response upon recognition of the fungal Avr2 and Avr5 determinants, respectively. Each resistance protein is comprised largely of leucine-rich repeats (LRRs) and the specificity of recognition is thought to occur through a particular domain. We have investigated this further using domain swaps between Cf-2 and Cf-5. Engineered chimeric genes containing portions of Cf-2 and Cf-5 were expressed and shown to be functional. The results clearly show that the specificity for the particular avirulence determinant is restricted to a region of each gene that encodes a subset of LRRs containing the highest level of intergenic variability. In addition, two non-functional mutants of Cf-5 were characterized and their significance discussed.
199-202
Seear, Paul J.
251e9ee4-3fa2-42a4-acef-f03d06a5d90c
Dixon, Mark S.
9debc1f1-fae6-4ed4-a9b5-f080577c15fc
2003
Seear, Paul J.
251e9ee4-3fa2-42a4-acef-f03d06a5d90c
Dixon, Mark S.
9debc1f1-fae6-4ed4-a9b5-f080577c15fc
Seear, Paul J. and Dixon, Mark S.
(2003)
Variable leucine-rich repeats of tomato disease resistance genes Cf-2
and Cf-5 determine specificity.
Molecular Plant Pathology, 4 (3), .
(doi:10.1046/j.1364-3703.2003.00162.x).
Abstract
The tomato Cf-2 and Cf-5 genes confer race specific resistance to infection by the leaf mould pathogen Cladosporium fulvum. The encoded proteins induce a defence response upon recognition of the fungal Avr2 and Avr5 determinants, respectively. Each resistance protein is comprised largely of leucine-rich repeats (LRRs) and the specificity of recognition is thought to occur through a particular domain. We have investigated this further using domain swaps between Cf-2 and Cf-5. Engineered chimeric genes containing portions of Cf-2 and Cf-5 were expressed and shown to be functional. The results clearly show that the specificity for the particular avirulence determinant is restricted to a region of each gene that encodes a subset of LRRs containing the highest level of intergenic variability. In addition, two non-functional mutants of Cf-5 were characterized and their significance discussed.
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Published date: 2003
Organisations:
Biological Sciences
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Local EPrints ID: 40511
URI: http://eprints.soton.ac.uk/id/eprint/40511
ISSN: 1464-6722
PURE UUID: f46ca674-f815-41bd-bc8e-9f2dbb414777
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Date deposited: 04 Jul 2006
Last modified: 15 Mar 2024 08:20
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Author:
Paul J. Seear
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