Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts
Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts
The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling.
115-122
Bhome, Rahul
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Goh, Rebecca
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Pickard, Karen
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Mellone, Massimiliano
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Sayan, A. Emre
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Mirnezami, Alex
b3c7aee7-46a4-404c-bfe3-f72388e0bc94
2017
Bhome, Rahul
d7b1e0d3-5925-460a-871d-5f52f69c649b
Goh, Rebecca
2dbe79e1-ec3d-4f2f-86bf-70f33269eab8
Pickard, Karen
e5188669-dff1-49c7-9c6f-8122b0c74bd9
Mellone, Massimiliano
b0301b32-14f8-4203-9026-b7f90885cab9
Sayan, A. Emre
d1dbbcad-9c53-47c1-8b7e-1b45cc56e077
Mirnezami, Alex
b3c7aee7-46a4-404c-bfe3-f72388e0bc94
Bhome, Rahul, Goh, Rebecca, Pickard, Karen, Mellone, Massimiliano, Sayan, A. Emre and Mirnezami, Alex
(2017)
Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts.
In,
Rani, S.
(ed.)
MicroRNA Profiling.
(Methods in Molecular Biology, 1509)
New York.
Humana, .
(doi:10.1007/978-1-4939-6524-3_11).
Record type:
Book Section
Abstract
The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling.
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More information
e-pub ahead of print date: 9 November 2016
Published date: 2017
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 405386
URI: http://eprints.soton.ac.uk/id/eprint/405386
PURE UUID: 8d637a28-1361-427e-8d1e-a525c260f4f5
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Date deposited: 03 Feb 2017 11:26
Last modified: 16 Mar 2024 04:14
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Contributors
Author:
Rebecca Goh
Author:
Karen Pickard
Author:
Massimiliano Mellone
Editor:
S. Rani
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